Application of pseudo-two phase partitioning bioreactor (P-TPPB) to the production of biodiesel

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• 作者：Young Wook Go (1)
  Sung Ho Yeom (1)
  
• 关键词：Pseudo ; two phase partitioning bioreactor ; Enzyme ; Biodiesel ; Methanol ; Inhibition
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：37
• 期：2
• 页码：269-275
• 全文大小：433 KB
• 作者单位：Young Wook Go (1)
  Sung Ho Yeom (1)
  
  1. Department of Biochemical Engineering, Gangneung-Wonju National University, Gangneung, Gangwon-do, 210-702, Korea
  
• ISSN：1615-7605

文摘

Pseudo-two phase partitioning bioreactor (P-TPPB) was newly proposed as an extension of the application of TPPB to bioprocesses in which hydrophilic substrates and/or products are involved. The feasibility of P-TPPB was demonstrated in enzymatic biodiesel production, where methanol completely inhibits the enzymes. Unlike conventional TPPB, the P-TPPB comprises a hydrophobic first phase (soybean oil) and hydrophilic second phase. n-Pentanol was found to be the optimum for the second phase, since P-TPPB containing n-pentanol showed the greatest total biodiesel conversion and highest fatty acid methyl ester content. The enzyme was repeatedly used to produce biodiesel in P-TPPB, while maintaining its activity at over 95?% relative to that of the intact enzyme.