Polydimethylsiloxane template-based size-selective assembly of single biological cells
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- 作者：Jae-Hyoung Park ; Seung-Ki Lee ; Sunghwan Jung
- 关键词：Templated assembly by selective removal ; Biological cells ; Polydimethylsiloxane ; Ultrasonic ; Transparent and biocompatible template ; Optical cell diagnostics
- 刊名：BioChip Journal
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：8
- 期：4
- 页码：254-259
- 全文大小：372 KB
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- 刊物主题：Chemistry
Biotechnology - 出版者：The Korean BioChip Society, in co-publication with Springer Verlag GmbH
- ISSN：2092-7843
文摘
This paper presents experimental demonstrations of size-selective parallel templated assembly of single biological cells on a polydimethylsiloxane (PDMS) template. Using the ultrasonic assembly technique known as templated assembly by selective removal (TASR), single chlorella cells with diameters between 5 and 20 μm were individually assembled in parallel with high size-based selectivity and precise alignment into their size-matching hemispherical assembly sites on the template. Optical observation of assembled single cells on the template without cell staining was enabled by transparency of the PDMS template. No significant change in the cell morphology was observed after assembly, confirming that the conditions of ultrasound used in the assembly are compatible. The presented size-selective trapping of single biological cells on the transparent and biocompatible PDMS template represents a biological tool with potential for direct implementation in various biological applications including cell sorting and isolation for optical cell diagnostics or cell templating for artificial tissue replication.