Effect of nitrogen and phosphorus fertilization on the composition of rhizobacterial communities of two Chilean Andisol pastures

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• 作者：Milko A. Jorquera (1)
  Oscar A. Martínez (2)
  Luis G. Marileo (3)
  Jacquelinne J. Acu?a (3)
  Surinder Saggar (4)
  María L. Mora (1)
  
• 关键词：Bacterial community ; Fertilization ; Pasture ; Rhizosphere ; Andisol
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：30
• 期：1
• 页码：99-107
• 全文大小：473 KB
• 作者单位：Milko A. Jorquera (1)
  Oscar A. Martínez (2)
  Luis G. Marileo (3)
  Jacquelinne J. Acu?a (3)
  Surinder Saggar (4)
  María L. Mora (1)
  
  1. Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Ave. Francisco Salazar, 01145, Temuco, Chile
  2. Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Campus Isla Tejas s/n, Valdivia, Chile
  3. Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Temuco, Chile
  4. Ecosystems and Global Change, Landcare Research, Palmerston North, 4442, New Zealand
  
• ISSN：1573-0972

文摘

The effect of nitrogen (N) and phosphorus (P) fertilization on composition of rhizobacterial communities of volcanic soils (Andisols) from southern Chile at molecular level is poorly understood. This paper investigates the composition of rhizobacterial communities of two Andisols under pasture after 1- and 6-year applications of N (urea) and P (triple superphosphate). Soil samples were collected from two previously established sites and the composition of rhizobacterial communities was determined by denaturing gradient gel electrophoresis (PCR–DGGE). The difference in the composition and diversity between rhizobacterial communities was assessed by nonmetric multidimensional scaling (MDS) analysis and the Shannon–Wiener index. In Site 1 (fertilized for 1?year), PCR–DGGE targeting 16S rRNA genes and MDS analysis showed that moderate N application (270?kg?N?ha??year?) without P significantly changed the composition of rhizobacterial communities. However, no significant community changes were observed with P (240?kg P?ha??year?) and N–P application (270?kg?N?ha??year? plus 240?kg P?ha??year?). In Site 2 (fertilized for 6?years with P; 400?kg P?ha??year?), PCR–DGGE targeting rpoB, nifH, amoA and alkaline phosphatase genes and MDS analysis showed changes in rhizobacterial communities only at the highest rate of N application (600?kg?N?ha??year?). Quantitative PCR targeting 16S rRNA genes also showed higher abundance of bacteria at higher N application. In samples from both sites, the Shannon–Wiener index did not show significant difference in the diversity of rhizobacterial communities. The changes observed in rhizobacterial communities coincide in N fertilized pastures with lower soil pH and higher pasture yields. This study indicates that N–P application affects the soil bacterial populations at molecular level and needs to be considered when developing fertilizer practices for Chilean pastoral Andisols.