Tri-layered silk fibroin and poly-?-caprolactone small diameter vascular grafts tested in vitro and in vivo

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• 作者：A. Reum Park ; Young-Hwan Park ; Hyun Jeong Kim ; Min-Keun Kim…
• 关键词：silk fibroin ; poly ; ε ; caprolactone ; nanofiber ; tri ; layers ; scaffold ; vascular tissue engineering
• 刊名：Macromolecular Research
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：23
• 期：10
• 页码：924-936
• 全文大小：982 KB
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• 作者单位：A. Reum Park (1)
  Young-Hwan Park (1)
  Hyun Jeong Kim (2)
  Min-Keun Kim (3)
  Seong-Gon Kim (3)
  HaeYong Kweon (4)
  Subhas C. Kundu (5)
  
  1. Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 151-921, Korea
  2. Department of Dental Anesthesiology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 110-768, Korea
  3. Department of Oral and Maxillofacial Surgery, Gangneung-Wonju National University, Gangneung, Gangwon, 210-702, Korea
  4. Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, Jeonju, Jeonbuk, 560-500, Korea
  5. Department of Biotechnology, Indian Institute of Technology Kharagpur (IIT), West Bengal, 721302, India
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Physical Chemistry
  Polymer Sciences
  Characterization and Evaluation of Materials
  Soft and Granular Matter, Complex Fluids and Microfluidics
  Nanochemistry
  Nanotec
  
• 出版者：The Polymer Society of Korea, co-published with Springer
• ISSN：2092-7673

文摘

A silk fibroin (SF) and poly-ε-caprolactone (PCL) tri-layered nano-fibrous scaffold composed of an inner, middle, and outer layer is fabricated via sequential electrospinning. The middle layer of the SF/PCL blend nano-fiber is introduced to minimize delamination of each layer of the scaffold. The inner layer is composed of SF with Spirulina maxima extract (SP), and the outer is composed of PCL. The anti-thrombic effects of SP are tested first. The structure, mechanical properties, and cytocompatibility of the scaffold are evaluated. The tri-layered nano-fiber scaffold is implanted into a rat carotid artery, and the sample after 3 weeks of implantation is evaluated histologically. The SP exerts anti-thrombic activity, and the SF with SP inhibits platelet adhesion. The tri-layered scaffold with the middle layer composed of SF/PCL blend exhibits excellent tensile strengths, burst pressure strength, and suture retention strength. The fabricated material does not induce any cytotoxicity. The cells are well spread on the scaffold. Recipient vessel maintains patency 3 weeks after implantation. The inner lumen of the scaffold reveals regenerated endothelial cells. The results indicate that the tri-layered tubular SF/PCL vascular grafts can be used in vascular tissue engineering due to their excellent mechanical properties and good tissue regeneration capability.