Determination of Ethylenediaminetetraacetic Acid from Swine Organs, Feces, Urine, and Plasma by Liquid Chromatography Tandem Mass Spectrometry

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• 作者：Agnes Meszaros ; Jeno Fekete
• 关键词：EDTA ; LC/MS/MS ; Biological samples ; Validation ; Determination
• 刊名：Food Analytical Methods
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：8
• 期：3
• 页码：678-684
• 全文大小：570 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

The study describes the development of a method for determination of ethylenediaminetetraacetic acid (EDTA) in various biological matrices after extraction as sample preparation using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The method is based on the complex formation of the excess of EDTA with FeIII. The excretion of EDTA in swine, treated with high doses of EDTA against dysentery, was monitored over a week by measuring its concentration in urine, plasma, and feces after stopping the administration of the medication. After slaughter, the EDTA concentration was measured also in the fatty tissue, kidney, liver, and muscle in order to determine the distribution of its residue between the tissues. The new method is fast, and the one-step sample preparation procedure may be regarded as derivatization. The method is applicable for low-concentration analysis with 3.0?ng?mL? limit of detection and 10.0?ng?mL? limit of quantification, while the relative standard deviation does not exceed 4.3?%.