Rapid determination of phenylethanolamine?A in biological samples by enzyme-linked immunosorbent assay and lateral-flow immunoassay

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• 作者：Xiangmei Li ; Wenjun Wang ; Limiao Wang ; Qi Wang…
• 关键词：β ; Adrenergic agonist ; Phenylethanolamine?A ; Indirect competitive ELISA ; Lateral ; flow immunoassay ; Biological sample
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：407
• 期：25
• 页码：7615-7624
• 全文大小：649 KB
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• 作者单位：Xiangmei Li (1) (2)
  Wenjun Wang (2)
  Limiao Wang (1)
  Qi Wang (1)
  Xingyao Pei (1)
  Haiyang Jiang (1)
  
  1. College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China
  2. Beijing WDWK Biotechnology Company, Ltd., No. 156 Beiqing Road, Beijing, 100085, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Phenylethanolamine A (PA) is a β-adrenergic agonist, which was first used in animal husbandry as a growth promoter in China in 2010. In this study, a monoclonal-antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (icELISA) and lateral-flow immunoassay (LFA) for the detection of PA in swine urine and pork were developed. The immunogen was prepared by linking PA hapten with carrier protein via a diazotization method. The IC₅₀ value of the optimized icELISA was 0.44 ng mL?. The limits of detection of the icELISA for PA in swine urine and pork were 0.13 ng mL? and 0.39 ng g?, respectively. The recoveries of PA from spiked swine urine and pork were in the range 82.0-07.4 % and 81.8-13.3 %, respectively, with the coefficients of variation in the range 4.1-6.2 % and 1.2-.3 %, respectively. The mAbs had negligible cross reactivity with 10 other β-agonists. In contrast, the LFA had a cut-off level of 5 ng mL? (g) in swine urine and pork, and the results could be achieved within 5 min. Ten blind samples of swine urine were analyzed simultaneously by icELISA, LFA, and ultra-high-performance liquid chromatography–tandem mass spectrometry, and the results of the three methods agreed well. Therefore, the combination of two immunoassays provides an effective and rapid screening method for detection of PA residues in biological samples.