Sterile-filtered saliva is a strong inducer of IL-6 and IL-8 in oral fibroblasts

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• 作者：Barbara Cvikl (1) (2) (3)
  Adrian Lussi (1)
  Andreas Moritz (3)
  Anton Sculean (4)
  Reinhard Gruber (1) (2) (4)
  
  1. Department of Preventive ; Restorative and Pediatric Dentistry ; School of Dental Medicine ; University of Bern ; Bern ; Switzerland
  2. Laboratory of Oral Cell Biology ; School of Dental Medicine ; University of Bern ; Bern ; Switzerland
  3. Department of Conservative Dentistry and Periodontology ; Medical University of Vienna ; Vienna ; Austria
  4. Department of Periodontology ; School of Dental Medicine ; University of Bern ; Bern ; Switzerland
  
• 关键词：Saliva ; Inflammation ; Fibroblasts ; Cytokines ; NF魏B ; Microarray ; Luminex technology
• 刊名：Clinical Oral Investigations
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：19
• 期：2
• 页码：385-399
• 全文大小：1,792 KB
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• 刊物类别：Medicine
• 刊物主题：Dentistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1436-3771

文摘

Objectives Saliva has been implicated to support oral wound healing, a process that requires a transient inflammatory reaction. However, definitive proof that saliva can provoke an inflammatory response remained elusive. Materials and methods We investigated the ability of freshly harvested and sterile-filtered saliva to cause an inflammatory response of oral fibroblasts and epithelial cells. The expression of cytokines and chemokines was assessed by microarray, RT-PCR, immunoassays, and Luminex technology. The involvement of signaling pathways was determined by Western blot analysis and pharmacologic inhibitors. Results We report that sterile-filtered whole saliva was a potent inducer of IL-6 and IL-8 in fibroblasts from the gingiva, the palate, and the periodontal ligament, but not of oral epithelial cells. This strong inflammatory response requires nuclear factor-kappa B and mitogen-activated protein kinase signaling. The pro-inflammatory capacity is heat stable and has a molecular weight of Toll-like receptor signaling pathway. Conclusions We conclude that saliva can provoke a robust inflammatory response in oral fibroblasts involving the classical nuclear factor-kappa B and mitogen-activated protein kinase signaling pathway. Clinical relevance Since fibroblasts but not epithelial cells show a strong inflammatory response, saliva may support the innate immunity of defect sites exposing the oral connective tissue.