High extracellular K+ evokes changes in voltage-dependent K+ and Na+ currents and volume regulation in astrocytes
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- 作者：Helena Neprasova (1) (2)
Miroslava Anderova (1) (2)
David Petrik (4)
Lydia Vargova (2) (3)
Sarka Kubinova (2) (3)
Alexandr Chvatal (1) (2)
Eva Sykova (2) (3) - 关键词：Astrogliosis ; GFAP ; Patch ; clamp ; Swelling ; Hypotonic stress ; 3D ; morphometry
- 刊名：Pfl眉gers Archiv - European Journal of Physiology
- 出版年：2007
- 出版时间：March 2007
- 年：2007
- 卷：453
- 期：6
- 页码：839-849
- 全文大小：538KB
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Miroslava Anderova (1) (2)
David Petrik (4)
Lydia Vargova (2) (3)
Sarka Kubinova (2) (3)
Alexandr Chvatal (1) (2)
Eva Sykova (2) (3)
1. Department of Neurobiology, Institute of Experimental Medicine, The Academy of Sciences of the Czech Republic, Videnska 1083, Prague, 4-142 20, Czech Republic
2. Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Second Medical Faculty, Charles University, V Uvalu 84, Prague, 5-150 06, Czech Republic
4. Department of Physiology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229-3900, USA
3. Department of Neuroscience, Institute of Experimental Medicine, The Academy of Sciences of the Czech Republic, Videnska 1083, Prague, 4-142 20, Czech Republic - ISSN：1432-2013
文摘
[K+]e increase accompanies many pathological states in the CNS and evokes changes in astrocyte morphology and glial fibrillary acidic protein expression, leading to astrogliosis. Changes in the electrophysiological properties and volume regulation of astrocytes during the early stages of astrocytic activation were studied using the patch-clamp technique in spinal cords from 10-day-old rats after incubation in 50?mM K+. In complex astrocytes, incubation in high K+ caused depolarization, an input resistance increase, a decrease in membrane capacitance, and an increase in the current densities (CDs) of voltage-dependent K+ and Na+ currents. In passive astrocytes, the reversal potential shifted to more positive values and CDs decreased. No changes were observed in astrocyte precursors. Under hypotonic stress, astrocytes in spinal cords pre-exposed to high K+ revealed a decreased K+ accumulation around the cell membrane after a depolarizing prepulse, suggesting altered volume regulation. 3D confocal morphometry and the direct visualization of astrocytes in enhanced green fluorescent protein/glial fibrillary acidic protein mice showed a smaller degree of cell swelling in spinal cords pre-exposed to high K+ compared to controls. We conclude that exposure to high K+, an early event leading to astrogliosis, caused not only morphological changes in astrocytes but also changes in their membrane properties and cell volume regulation.