Whole body creatine and protein kinetics in healthy men and women: effects of creatine and amino acid supplementation
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- 作者：Satish C. Kalhan ; Lourdes Gruca ; Susan Marczewski ; Carole Bennett…
- 关键词：Creatine ; Guanidinoacetic acid ; Glycine ; Phenylalanine ; Stable isotopes ; Kinetics
- 刊名：Amino Acids
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：48
- 期：3
- 页码：677-687
- 全文大小：567 KB
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Lourdes Gruca (1)
Susan Marczewski (1)
Carole Bennett (1)
China Kummitha (3)
1. Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, NE-40, 9500 Euclid Av, Cleveland, OH, 44195, USA
2. Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, USA
3. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Biochemistry
Analytical Chemistry
Biochemical Engineering
Life Sciences
Proteomics
Neurobiology - 出版者：Springer Wien
- ISSN：1438-2199
文摘
Creatine kinetics were measured in young healthy subjects, eight males and seven females, age 20-30 years, after an overnight fast on creatine-free diet. Whole body turnover of glycine and its appearance in creatine was quantified using [1-13C] glycine and the rate of protein turnover was quantified using L-ring [2H5] phenylalanine. The creatine pool size was estimated by the dilution of a bolus [C2H3] creatine. Studies were repeated following a five days supplement creatine 21 g.day−1 and following supplement amino acids 14.3 g day−1. Creatine caused a ten-fold increase in the plasma concentration of creatine and a 50 % decrease in the concentration of guanidinoacetic acid. Plasma amino acids profile showed a significant decrease in glycine, glutamine, and taurine and a significant increase in citrulline, valine, lysine, and cysteine. There was a significant decrease in the rate of appearance of glycine, suggesting a decrease in de-novo synthesis (p = 0.006). The fractional and absolute rate of synthesis of creatine was significantly decreased by supplemental creatine. Amino acid supplement had no impact on any of the parameters. This is the first detailed analysis of creatine kinetics and the effects of creatine supplement in healthy young men and women. These methods can be applied for the analysis of creatine kinetics in different physiological states. Keywords Creatine Guanidinoacetic acid Glycine Phenylalanine Stable isotopes Kinetics