Activation of Villous Trophoblastic p38 and ERK1/2 Signaling Pathways in Preterm Preeclampsia and HELLP Syndrome

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• 作者：Szilvia Szabo ; Meera Mody ; Roberto Romero ; Yi Xu…
• 关键词：Anti ; angiogenic factor ; Hypertension ; Mitogen activated protein kinase ; Oxidative stress ; Pregnancy ; Signal transduction ; Syncytiotrophoblast
• 刊名：Pathology & Oncology Research
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：21
• 期：3
• 页码：659-668
• 全文大小：1,261 KB
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• 作者单位：Szilvia Szabo (1) (2)
  Meera Mody (3) (4)
  Roberto Romero (3)
  Yi Xu (3)
  Katalin Karaszi (2)
  Noemi Mihalik (2) (5)
  Zhonghui Xu (3)
  Gaurav Bhatti (3)
  Tibor Fule (2)
  Petronella Hupuczi (6) (7)
  Tibor Krenacs (2)
  Janos Rigo Jr. (6)
  Adi L. Tarca (3) (8)
  Sonia S. Hassan (3) (9)
  Tinnakorn Chaiworapongsa (3) (9)
  Ilona Kovalszky (2)
  Zoltan Papp (6) (7)
  Nandor Gabor Than (10) (2) (3) (6) (7) (9)
  
  1. Department of Morphology and Physiology, Faculty of Health Sciences, Semmelweis University, Budapest, Hungary
  2. First Department of Pathology and Experimental Cancer Research, Faculty of Medicine, Semmelweis University, Ulloi Street 26, 1085, Budapest, Hungary
  3. Perinatology Research Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, 3990 John R, Detroit, MI, 48201, USA
  4. University of Chicago, Chicago, IL, USA
  5. Department of Dermatology, Dermatooncology and Venerology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
  6. First Department of Obstetrics and Gynecology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
  7. Maternity Private Department, Kutvolgyi Clinical Block, Faculty of Medicine, Semmelweis University, Budapest, Hungary
  8. Center for Molecular Medicine and Genetics, School of Medicine, Wayne State University, Detroit, MI, USA
  9. Department of Obstetrics and Gynecology, School of Medicine, Wayne State University, Detroit, MI, USA
  10. Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary
  
• 刊物主题：Cancer Research; Oncology; Pathology; Immunology; Biomedicine general;
• 出版者：Springer Netherlands
• ISSN：1532-2807

文摘

Preterm preeclampsia is associated with the failure of trophoblast invasion, placental hypoxic/ischemic injury and the release of toxic substances, which promote the terminal pathway of preeclampsia. In term preeclampsia, factors yet unknown trigger the placenta to induce the terminal pathway. The contribution of the villous trophoblast to these pathologic events has not been fully elucidated. Here we aimed to study how stress and signaling pathways influence trophoblastic functions in various subforms of preeclampsia. Tissue microarrays (TMAs) were constructed from placentas obtained from pregnant women in the following groups: 1-) preterm preeclampsia with (n--) or without (n--) HELLP syndrome; 3) late-onset preeclampsia (n--); 4-) preterm (n--) and term (n--) controls. TMA slides were stained for phosphorylated Akt-1, ERK1/2, JNK, and p38 kinases, and trophoblastic immunostainings were semi-quantitatively evaluated. BeWo cells were kept in various stress conditions, and the expression of FLT1, GCM1, LEP, and PGF was profiled by qRT-PCR, while Akt-1, ERK1/2, JNK, and p38 kinase activities were measured with phospho-kinase immunoassays. We found that: 1) Placental LEP and FLT1 expression was up-regulated in preterm preeclampsia with or without HELLP syndrome compared to controls; 2) Mean pp38 immunoscore was higher in preterm preeclampsia, especially in cases with HELLP syndrome, than in controls. 3) Mean pERK1/2 immunoscore was higher in preterm preeclampsia with HELLP syndrome than in controls. 4) In BeWo cells, ischemia up-regulated LEP expression, and it increased JNK and decreased ERK1/2 activity. 5) Hypoxia up-regulated FLT1 and down-regulated PGF expression, and it increased ERK1/2, JNK and p38 activity. 6) IL-1β treatment down-regulated PGF expression, and it increased JNK and p38 activity. 7) The p38 signaling pathway had the most impact on LEP, FLT1 and PGF expression. In conclusion, hypoxic and ischemic stress, along with unknown factors, activates trophoblastic p38 signaling, which has a key role in villous trophoblastic functional changes in preterm preeclampsia. The activation of ERK1/2 signaling may induce additional trophoblastic functional changes in HELLP syndrome, while distinct mechanisms may promote late-onset preeclampsia.