Differential cytotoxicity and sonosensitization by sanazole: effect of cell type and acoustic parameters
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- 作者：Mariame A. Hassan (1) (2)
Yukihiro Furusawa (1)
Qing-Li Zhao (1)
Ichiro Takasaki (3)
Loreto B. Feril Jr. (4)
Katsuro Tachibana (4)
Nobuki Kudo (5)
Masami Minemura (6)
Toshiro Sugiyama (6)
Takashi Kondo (1) - 关键词：Apoptosis ; Pulse repetition frequency ; Sanazole ; Sonosensitizer ; Ultrasound
- 刊名：Journal of Medical Ultrasonics
- 出版年：2011
- 出版时间：April 2011
- 年：2011
- 卷：38
- 期：2
- 页码：65-72
- 全文大小：520KB
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Yukihiro Furusawa (1)
Qing-Li Zhao (1)
Ichiro Takasaki (3)
Loreto B. Feril Jr. (4)
Katsuro Tachibana (4)
Nobuki Kudo (5)
Masami Minemura (6)
Toshiro Sugiyama (6)
Takashi Kondo (1)
1. Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan
2. Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr Al-Aini str., 11562, Cairo, Egypt
3. Division of Molecular Genetic Research, Life Science Research Center, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan
4. Department of Anatomy, School of Medicine, Fukuoka University, Nanakuma, Fukuoka, 814-0180, Japan
5. Laboratory of Biomedical Instrumentation and Measurement, Graduate School of Information Science and Technology, Hokkaido University, Sapporo, 060-0814, Japan
6. Department of Gastroenterology and Hematology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan
文摘
Purpose Although sanazole has been used as a hypoxic radiosensitizer, we recently reported on its ability to sensitize U937 cells to hyperthermia and X-irradiation under aerobic conditions, enhancing apoptotic cell death following the combined treatment. The current study was undertaken to evaluate the effect of sanazole as a sonosensitizer under previously studied acoustic conditions of different pulse repetition frequencies, using two cell lines representative of solid tumours and haematopoietic cancers. Methods Cells were treated with different doses of sanazole. Flow-cytometric analysis and DNA fragmentation assay were carried out at different times, and morphological features were also inspected. For ultrasound treatment, cells were pre-incubated with a non-cytotoxic dose of sanazole for 30?min before exposure. Evaluation of cell killing and a parallel examination of intracellular oxidative stress levels in both cell lines were performed using flow cytometry. Results Sanazole alone displayed selective cytotoxic effects towards solid tumour-derived cancer cells, resulting in complete cell death after 24?h of treatment, and enhanced the ultrasound-induced cell killing 6?h post-treatment. The enhancement seemed to be mediated by an additive increase in intracellular oxidative stress levels. Conclusion Sanazole seems to be an efficient cytotoxic agent for the treatment of solid tumours and a promising sonosensitizer under aerobic conditions.