Morphological and molecular characterisation of Hemicaloosia guangzhouensis n. sp. (Nematoda: Caloosiidae) from China

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• 作者：Ke Wang ; Yu Li ; Hui Xie ; Wen-Jia Wu ; Chun-Ling Xu
• 关键词：Hemicaloosia ; Morphology ; Molecular ; ITS rRNA gene ; D2 ; D3 region of the 28S rRNA gene ; 18S rRNA gene
• 刊名：European Journal of Plant Pathology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：144
• 期：3
• 页码：497-507
• 全文大小：1,692 KB
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• 作者单位：Ke Wang (1)
  Yu Li (1)
  Hui Xie (1)
  Wen-Jia Wu (1)
  Chun-Ling Xu (1)
  
  1. Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

A new plant nematode species, Hemicaloosia guangzhouensis n. sp., collected from the soil associated with Schima superba in a forest park in Guangzhou, China is described. The new species is characterized by having a female with a slender body, 798–961 μm in length, stylet 59–69 μm long, tail 115–145 μm long, a membranous sheath closely adpressed to the entire body, lateral fields marked by one longitudinal line with breaks and anastomoses of transverse striae, total number of annuli 282–313, two lip annuli both projecting slightly anterior, excretory pore 51–58 annuli from anterior end, the number of annulus between vulva and anus 7–11, the number of annulus from vulva to tail terminus 58–69, spermatheca empty; and male unknown. The internal transcribed spacer of ribosomal RNA (ITS rRNA) gene, D2-D3 region of 28S ribosomal RNA (28S rRNA) gene, partial 18S ribosomal RNA (18S rRNA) gene of the new species were amplified and sequenced. The 50 % majority rule consensus trees inferred from ITS rRNA, 28S rRNA, partial 18S rRNA gene sequences of H. guangzhouensis n. sp. and some other species in the suborder Criconematina are also presented in this study. PCR-ITS-RFLP diagnostic profile generated by six restriction enzymes for H. guangzhouensis n. sp. is given. Keywords Hemicaloosia Morphology Molecular ITS rRNA gene D2-D3 region of the 28S rRNA gene 18S rRNA gene