Overexpression p21WAF1/CIP1 in suppressing retinal pigment epithelial cells and progression of proliferative vitreoretinopathy via inhibition CDK2 and cyclin E

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• 作者：Ying Wang (6)
  Zhigang Yuan (6)
  Caiyun You (6)
  Jindong Han (6)
  Haiyan Li (7)
  Zhuhong Zhang (6)
  Hua Yan (6)
  
  6. Department of Ophthalmology ; Tianjin Medical University General Hospital ; Tianjin ; 300052 ; China
  7. Shanxi Eye Hospital ; Taiyuan City ; Shanxi Province ; 030002 ; China
  
• 关键词：P21Waf1/Cip1 ; Cell cycle arrest ; Retinal pigment epithelial cells ; Proliferative vitreoretinopathy
• 刊名：BMC Ophthalmology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：1,091 KB
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  36. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2415/14/144/prepub
  
• 刊物主题：Ophthalmology;
• 出版者：BioMed Central
• ISSN：1471-2415

文摘

Background P21 is one kind of cyclin-dependent kinase inhibitor that can prevent cells from going through the G1/S phase checkpoint and inhibit cell proliferation. Proliferative vitreoretinopathy (PVR) is a proliferative response in the eye. The aim of this study was to determine whether p21Waf1/Cip1 (p21) suppresses the proliferation and migration of retinal pigment epithelial (RPE) cells in vitro and controls PVR development in vivo. Methods Cell cycle analyses and transwell assays were conducted to assess cell proliferation characteristics and the migration ability of RPE cells after transfection with p21. Western blot and reverse-transcription polymerase chain reaction technologies were used to detect the expression of p21, CDK2 and cyclinE in RPE cells and rabbit retinal tissues. The impact of increasing p21 expression on PVR development was conducted by implantation of an adenovirus vector containing rabbit p21 (rAd-p21) in a PVR rabbit model. The prevalence of PVR and retinal detachment was determined by indirect ophthalmoscopy on days 3, 7, 14, and 21 after the injection of rAd-p21 into the vitreous. B scans and hematoxylin-eosin staining were employed to check rabbit retinas on day 21. Results Cell cycle analyses and transwell assays showed that p21 inhibited the proliferation and migration of RPE cells. Increased expression of p21 was detected in cultured RPE cells and rabbit retinas after transfection with the p21 gene, whereas levels of CDK2 and cyclinE were decreased. The increase in p21 expression effectively suppressed the development of PVR in a rabbit model. Conclusions The increase in p21 expression in RPE cells not only inhibits the proliferation and migration of RPE cells in vitro, but also suppresses the development of PVR in vivo, which indicates its therapeutic potential in treating PVR.