Green tea extract suppresses adiposity and affects the expression of lipid metabolism genes in diet-induced obese zebrafish
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- 作者：Takahiro Hasumura (1)
Yasuhito Shimada (2) (3) (4) (5)
Junya Kuroyanagi (2)
Yuhei Nishimura (2) (3) (4) (5)
Shinichi Meguro (1)
Yoshinori Takema (6)
Toshio Tanaka (2) (3) (4) (5) - 关键词：Body fat ; Catechin ; Diet ; induced obesity ; 3D micro ; computed tomography ; Green tea extract ; SOCS3 ; Zebrafish
- 刊名：Nutrition & Metabolism
- 出版年：2012
- 出版时间：December 2012
- 年：2012
- 卷：9
- 期：1
- 全文大小：340KB
- 参考文献：1. Malik VS, Popkin BM, Bray GA, Després JP, Hu FB: Sugar-sweetened beverages, obesity, type 2 diabetes mellitus, and cardiovascular disease risk. / Circulation 2010, 121:1356-364. CrossRef
2. Spiegelman BM, Flier JS: Obesity and the regulation of energy balance. / Cell 2001, 104:531-43. CrossRef
3. Ibrahim MM: Subcutaneous and visceral adipose tissue: structural and functional differences. / Obes Rev 2010, 11:11-8. CrossRef
4. Kuk JL, Katzmarzyk PT, Nichaman MZ, Church TS, Blair SN, Ross R: Visceral fat is an independent predictor of all-cause mortality in men. / Obesity 2006, 14:336-41. CrossRef
5. Rice-Evans CA, Miller NJ, Paganga G: Structure-antioxidant activity relationships of flavonoids and phenolic acids. / Free Rad Biol Med 1996, 20:933-56. CrossRef
6. Shankar S, Ganapathy S, Srivastava RK: Green tea polyphenols: biology and therapeutic implications in cancer. / Front Biosci 2007, 12:4881-899. CrossRef
7. Zaveri NT: Green tea and its polyphenolic catechins: medicinal uses in cancer and noncancer applications. / Life Sci 2006, 78:2073-080. CrossRef
8. Matsumoto N, Ishigaki F, Ishigaki A, Iwashina H, Hara Y: Reduction of blood glucose levels by tea catechin. / Biosci. Biotechnol. Biochem 1993, 57:525-27. CrossRef
9. Miura Y, Chiba T, Tomita I, Koizumi H, Miura S, Umegaki K, Hara Y, Ikeda M, Tomita T: Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice. / J. Nutr 2001, 131:27-2.
10. Klaus S, Pultz S, Thone-Reineke C, Wolfram S: Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. / Int J Obes 2005, 29:615-23. CrossRef
11. Murase T, Nagasawa A, Suzuki J, Hase T, Tokimitsu I: Beneficial effects of tea catechins on diet-induced obesity: stimulation of lipid catabolism in the liver. / Int J Obes 2002, 26:1459-464. CrossRef
12. Kozuma K, Chikama A, Hoshino E, Kataoka K, Mori K, Hase T, Katsuragi Y, Tokimitsu I, Nakamura H: Effect of intake of a beverage containing 540 mg catechins on the body composition of obese women and men. / Prog Med 2005, 25:1945-957.
13. Nagao T, Komine Y, Soga S, Meguro S, Hase T, Tanaka Y, Tokimitsu I: Ingestion of a tea rich in catechins leads to a reduction in body fat and malondialdehyde-modified LDL in men. / Am J Clin Nutr 2005, 81:122-29.
14. Nagao T, Hase T, Tokimitsu I: A green tea extract high in catechins reduces body fat and cardiovascular risks in humans. / Obesity 2007, 15:1473-483. CrossRef
15. Takase H, Nagao T, Otsuka K, Kozuma K, Kataoka K, Meguro S, Komikado M, Tokimitsu I: Effects of long-term ingestion of tea catechins on visceral fat accumulation and metabolic syndrome: pooling-analysis of 7 randomized controlled trials. / Jpn Pharmacol Ther 2008, 36:509-14.
16. Takeshita M, Takashima S, Harada U, Shibata E, Hosoya N, Takase H, Otsuka K, Meguro S, Komikado M, Tokimitsu I: Effects of long-term consumption of tea catechins-enriched beverage with no caffeine on body composition in humans. / Jpn Pharmacol Ther 2008, 36:767-76.
17. Zhang Y, Yu Y, Li X, Meguro S, Hayashi S, Katashima M, Yasumasu T, Wang J, Li K: Effects of catechin-enriched green tea beverage on visceral fat loss in adults with a high proportion of visceral fat: a double-blind, placebo-controlled, randomized trial. / J Funct Foods 2012, 4:315-22. CrossRef
18. Dulloo AG, Duret C, Rohrer D, Girardier L, Mensi N, Fathi M, Chantre P, Vandermander J: Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation in humans. / Am J Clin Nutr 1999, 70:1040-045.
19. Shimotoyodome A, Haramizu S, Inaba M, Murase T, Tokimitsu I: Exercise and green tea extract stimulate fat oxidation and prevent obesity in mice. / Med Sci Sports Exerc 2005, 37:1884-892. CrossRef
20. Furuyashiki T, Nagayasu H, Aoki Y, Bessho H, Hashimoto T, Kanazawa K, Ashida H: Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPARgamma2 and C/EBPalpha in 3 T3-L1 cells. / Biosci Biotech Biochem 2004, 68:2353-359. CrossRef
21. Ikeda I, Hamamoto R, Uzu K, Imaizumi K, Nagao K, Yanagita T, Suzuki Y, Kobayashi M, Kakuda T: Dietary gallate esters of tea catechins reduce deposition of visceral fat, hepatic triacylglycerol, and activities of hepatic enzymes related to fatty acid synthesis in rats. / Biosci Biotech Biochem 2005, 69:1049-053. CrossRef
22. Watanabe J, Kawabata J, Niki R: Isolation and identification of acetyl-CoA carboxylase inhibitors from green tea ( Camellia sinensis ). / Biosci Biotechnol Biochem 1998, 62:532-34. CrossRef
23. Best JD, Alderton WK: Zebrafish: an in vivo model for the study of neurological diseases. / Neuropsych Dis Treat 2008, 4:567-76. CrossRef
24. Kishi S, Slack BE, Uchiyama J, Zhdanova IV: Zebrafish as a genetic model in biological and behavioral gerontology: where development meets aging in vertebrates -a mini-review. / Gerontology 2009, 55:430-41. CrossRef
25. Lieschke GJ, Currie PD: Animal models of human disease: zebrafish swim into view. / Nat Rev Genet 2007, 8:353-67. CrossRef
26. Henderson RJ, Tocher DR: The lipid composition and biochemistry of freshwater fish. / Prog Lipid Res 1987, 26:281-47. CrossRef
27. H?ltt?-Vuori M, Salo VTV, Nyberg L, Brackmann C, Enejder A, Panula P, Ikonen E: Zebrafish: gaining popularity in lipid research. / Biochem J 2010, 429:235-42. CrossRef
28. Morais S, Knoll-Gellida A, André M, Barthe C, Babin PJ: Conserved expression of alternative splicing variants of peroxisomal acyl-CoA oxidase 1 in vertebrates and developmental and nutritional regulation in fish. / Physiol Genom 2007, 28:239-52. CrossRef
29. Song Y, Cone RD: Creation of a genetic model of obesity in a teleost. / FASEB J 2007, 21:2042-049. CrossRef
30. Stoletov K, Fang L, Choi SH, Hartvigsen K, Hansen LF, Hall C, Pattison J, Juliano J, Miller ER, Almazan F, Crosier P, Witztum JL, Klemke RL, Miller YI: Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish. / Circ Res 2009, 104:952-60. CrossRef
31. Oka T, Nishimura Y, Zang L, Hirano M, Shimada Y, Wang Z, Umemoto N, Kuroyanagi J, Nishimura N, Tanaka T: Diet-induced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. / BMC Physiol 2010, 10:21. CrossRef
32. Tainaka T, Shimada Y, Kuroyanagi J, Zang L, Oka T, Nishimura Y, Nishimura N, Tanaka T: Transcriptome analysis of anti-fatty liver action by Campari tomato using a zebrafish diet-induced obesity model. / Nutr Metab 2011, 8:88. CrossRef
33. Murase T, Haramizu S, Shimotoyodome A, Tokimitsu I, Hase T: Green tea extract improves running endurance in mice by stimulating lipid utilization during exercise. / Am J Physiol Reg Integr Comp Physiol 2006, 290:R1550-R1556. CrossRef
34. Westerfield M: / A guide for the laboratory use of zebrafish (Danio rerio). Eugene: University of Oregon Press; 2007.
35. Chen N, Bezzina R, Hinch E, Lewandowski PA, Cameron-Smith D, Mathai ML, Jois M, Sinclair AJ, Begg DP, Wark JD, Weisinger HS, Weisinger RS: Green tea, black tea, and epigallocatechin modify body composition, improve glucose tolerance, and differentially alter metabolic gene expression in rats fed a high-fat diet. / Nutr Res 2009, 29:784-93. CrossRef
36. Lee MS, Kim CT, Kim Y: Green tea (-)-epigallocatechin-3-gallate reduces body weight with regulation of multiple genes expression in adipose tissue of diet-induced obese mice. / Ann Nutr Metab 2009, 54:151-57. CrossRef
37. Sae-Tan S, Grove KA, Lambert JD: Weight control and prevention of metabolic syndrome by green tea. / Pharmacol Res 2011, 64:146-54. CrossRef
38. Park HJ, DiNatale DA, Chung MY, Park YK, Lee JY, Koo SI, O’Connor M, Manautou JE, Bruno RS: Green tea extract attenuates hepatic steatosis by decreasing adipose lipogenesis and enhancing hepatic antioxidant defenses in ob/ob mice. / J Nutr Biochem 2011, 22:393-00. CrossRef
39. Letexier D, Pinteur C, Large V, Fréring V, Beylot M: Comparison of the expression and activity of the lipogenic pathway in human and rat adipose tissue. / J Lipid Res 2003, 44:2127-134. CrossRef
40. Howard JK, Cave BJ, Oksanen LJ, Tzameli I, Bjo?rb?k C, Flier JS: Enhanced leptin sensitivity and attenuation of diet-induced obesity in mice with haploinsufficiency of Socs3. / Nat Med 2004, 10:734-38. CrossRef
41. Rieusset J, Bouzakri K, Chevillotte E, Ricard N, Jacquet D, Bastard JP, Laville M, Vidal H: Suppressor of cytokine signaling 3 expression and insulin resistance in skeletal muscle of obese and type 2 diabetic patients. / Diabetes 2004, 53:2232-241. CrossRef
42. Kanatani Y, Usui I, Ishizuka K, Bukhari A, Fujisaka S, Urakaze M, Haruta T, Kishimoto T, Naka T, Kobayashi M: Effects of pioglitazone on suppressor of cytokine signaling 3 expression: potential mechanisms for its effects on insulin sensitivity and adiponectin expression. / Diabetes 2007, 56:795-03. CrossRef
43. Gu H, Liu L, Ma S, Liu Y, Ren Y, Zhai L, Yu F, An L, Yang J: Inhibition of SOCS-3 in adipocytes of rats with diet-induced obesity increases leptin-mediated fatty acid oxidation. / Endocrine 2009, 36:546-54. CrossRef
44. Cnop M, Havel PJ, Utzschneider KM, Carr DB, Sinha MK, Boyko EJ, Retzlaff BM, Knopp RH, Brunzell JD, Kahn SE: Relationship of adiponectin to body fat distribution, insulin sensitivity and plasma lipoproteins: evidence for independent roles of age and sex. / Diabetologia 2003, 46:459-69.
45. Matsuzawa Y, Nakamura T, Takahashi M, Ryo M, Inoue S, Ikeda Y, Ohno M, Sakata T, Fukagawa K, Saitoh Y, Sato Y, Shirai K, Miyazaki S, Tokunaga K, Yamanouchi K, Takahashi M, Shibasaki T, Nagai M: New criteria for ‘obesity disease-in Japan. / Circ J 2002, 66:987-92. CrossRef
46. Judex S, Luu YK, Ozcivici E, Adler B, Lublinsky S, Rubin CT: Quantification of adiposity in small rodents using micro-CT. / Methods 2010, 50:14-9. CrossRef
47. Luu YK, Lublinsky S, Ozcivici E, Capilla E, Pessin JE, Rubin CT, Judex S: In vivo quantification of subcutaneous and visceral adiposity by micro-computed tomography in a small animal model. / Med Eng Phys 2009, 31:34-1. CrossRef
48. Folkestad A, Wold JP, R?rvik KA, Tschudi J, Haugholt KH, Kolstad K, M?rk?re T: Rapid and non-invasive measurements of fat and pigment concentrations in live and slaughtered Atlantic salmon (Salmo salar L.). / Aquaculture 2008, 280:129-35. CrossRef
49. Nanton DA, Vegusdal A, R?r? AMB, Ruyter B, Baeverfjord G, Torstensen BE: Muscle lipid storage pattern, composition, and adipocyte distribution in different parts of Atlantic salmon ( Salmo salar ) fed fish oil and vegetable oil. / Aquaculture 2007, 265:230-43. CrossRef
50. Romvári R, Hancz CS, Petrási ZS, Molnár T, Horn P: Non-invasive measurement of fillet composition of four freshwater fish species by computer tomography. / Aquacult Int 2002, 10:231-40. CrossRef - 作者单位：Takahiro Hasumura (1)
Yasuhito Shimada (2) (3) (4) (5)
Junya Kuroyanagi (2)
Yuhei Nishimura (2) (3) (4) (5)
Shinichi Meguro (1)
Yoshinori Takema (6)
Toshio Tanaka (2) (3) (4) (5)
1. Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tochigi, 321-3497, Japan
2. Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan
3. Department of Bioinformatics, Mie University Life Science Research Center, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan
4. Department of Omics Medicine, Mie University Industrial Technology Innovation Institute, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan
5. Mie University Medical Zebrafish Research Center, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan
6. Research and Development, Kao Corporation, 2-1-3 Bunka, Sumida-ku, Tokyo, 131-8501, Japan - ISSN：1743-7075
文摘
Background Visceral fat accumulation is one of the most important predictors of mortality in obese populations. Administration of green tea extract (GTE) can reduce body fat and reduce the risk of obesity-related diseases in mammals. In this study, we investigated the effects and mechanisms of GTE on adiposity in diet-induced obese (DIO) zebrafish. Methods Zebrafish at 3.5 to 4.5?months post-fertilization were allocated to four groups: non-DIO, DIO, DIO--.0025%GTE, and DIO--.0050%GTE. The non-DIO group was fed freshly hatched Artemia once daily (5?mg cysts/fish daily) for 40?days. Zebrafish in the three DIO groups were fed freshly hatched Artemia three times daily (60?mg cysts/fish daily). Zebrafish in the DIO--.0025%GTE and DIO--.0050%GTE groups were exposed to GTE after the start of feeding three times daily for 40?days. Results Three-dimensional microcomputed tomography analysis showed that GTE exposure significantly decreased the volume of visceral but not subcutaneous fat tissue in DIO zebrafish. GTE exposure increased hepatic expression of the lipid catabolism genes ACOX1 (acyl-coenzyme A oxidase 1, palmitoyl), ACADM (acyl-coenzyme A dehydrogenase, c-4 to c-12 straight chain), and PPARA (peroxisome proliferator-activated receptor alpha). GTE exposure also significantly decreased the visceral fat expression of SOCS3 (suppressor of cytokine signaling 3b) which inhibits leptin signaling. Conclusions The present results are consistent with those seen in mammals treated with GTE, supporting the validity of studying the effects of GTE in DIO zebrafish. Our results suggest that GTE exerts beneficial effects on adiposity, possibly by altering the expression of lipid catabolism genes and SOCS3.