Behavior of tricellulin during destruction and formation of tight junctions under various extracellular calcium conditions
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- 作者：Akira Takasawa (1)
Takashi Kojima (1)
Takafumi Ninomiya (2)
Mitsuhiro Tsujiwaki (1)
Masaki Murata (1)
Satoshi Tanaka (1)
Norimasa Sawada (1) - 关键词：Tight junction ; Tricellulin ; Phosphorylation ; Extracellular calcium ; Barrier ; Fence ; Human pancreatic cancer cell line
- 刊名：Cell and Tissue Research
- 出版年：2013
- 出版时间：January 2013
- 年：2013
- 卷：351
- 期：1
- 页码：73-84
- 全文大小：959KB
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Takashi Kojima (1)
Takafumi Ninomiya (2)
Mitsuhiro Tsujiwaki (1)
Masaki Murata (1)
Satoshi Tanaka (1)
Norimasa Sawada (1)
1. Department of Pathology, Sapporo Medical University School of Medicine, S1, W17, Sapporo, 060-8556, Japan
2. Department of Anatomy, Sapporo Medical University School of Medicine, Sapporo, Japan - ISSN：1432-0878
文摘
Tricellulin is an important component of tricellular tight junctions (TJs) and is involved in the formation of tricellular contacts. However, little is known about its regulation during the assembly and disassembly of tricellular TJs. By using the well-differentiated pancreatic cancer cell line HPAC, which highly expresses tricellulin at tricellular contacts, we have investigated changes in the localization, expression and phosphorylation of tricellulin and in its TJ functions as a barrier and fence during the destruction and formation of TJs induced by changes in the extracellular calcium concentration. During both extracellular Ca2+ depletion caused by EGTA treatment and Ca2+ repletion after Ca2+ starvation, the expression of tricellulin increased in whole lysates and in Triton-X-100-insoluble fractions without any change in its mRNA. The increases in immunoreactivity revealed by Western blotting were prevented by alkaline phosphatase treatment. Immunoprecipitation assays showed that tricellulin was phosphorylated on threonine residues when it increased after Ca2+ depletion and repletion. In the early stage after Ca2+ repletion, tricellulin was expressed not only at tricellular contacts but also in the cytoplasm and at bicellular borders. In confocal laser microscopy, tricellulin was observed at the apical-most regions and basolateral membranes of tricellular contacts after Ca2+ repletion. Knockdown of tricellulin delayed the recovery of the barrier and fence functions after Ca2+ repletion. Thus, the dynamic behavior of tricellulin during the destruction and formation of TJs under various extracellular calcium conditions seems to be closely associated with the barrier and fence functions of TJs.