Chronological histological changes during bone regeneration on a non-crosslinked atelocollagen matrix

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• 作者：Ryosuke Kagawa (1) (2)
  Mitsunobu Kishino (1)
  Sunao Sato (1)
  Ken Ishida (1) (2)
  Yuzo Ogawa (1)
  Kazunori Ikebe (2)
  Kaori Oya (3)
  Takuya Ishimoto (4)
  Takayoshi Nakano (4)
  Yoshinobu Maeda (2)
  Toshihisa Komori (5)
  Satoru Toyosawa (1)
  
• 关键词：Atelocollagen ; Bone regeneration ; Osteoblast ; Bone matrix ; Immunohistochemistry
• 刊名：Journal of Bone and Mineral Metabolism
• 出版年：2012
• 出版时间：November 2012
• 年：2012
• 卷：30
• 期：6
• 页码：638-650
• 全文大小：1290KB
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• 作者单位：Ryosuke Kagawa (1) (2)
  Mitsunobu Kishino (1)
  Sunao Sato (1)
  Ken Ishida (1) (2)
  Yuzo Ogawa (1)
  Kazunori Ikebe (2)
  Kaori Oya (3)
  Takuya Ishimoto (4)
  Takayoshi Nakano (4)
  Yoshinobu Maeda (2)
  Toshihisa Komori (5)
  Satoru Toyosawa (1)
  
  1. Department of Oral Pathology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan
  2. Department of Prosthodontics, Gerodontology and Oral Rehabilitation, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan
  3. Division for Interdisciplinary Dentistry, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan
  4. Division of Materials and Manufacturing Science, Osaka University Graduate School of Engineering, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan
  5. Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8523, Japan
  
• ISSN：1435-5604

文摘

Cleavage of the antigenic telopeptide region from type I collagen yields atelocollagen, and this is widely used as a scaffold for bone regeneration combined with cells, growth factors, etc. However, neither the biological effect of atelocollagen alone or its contribution to bone regeneration has been well studied. We evaluated the chronological histological changes during bone regeneration following implantation of non-crosslinked atelocollagen (Koken Co., Ltd.) in rat calvarial defects. One week after implantation, osteogenic cells positive for runt-related transcription factor 2 (Runx2) and osteoclasts positive for tartrate-resistant acid phosphatase (TRAP) were present in the atelocollagen implant in the absence of bone formation. The number of Runx2-positive osteogenic cells and Osterix-positive osteoblasts increased 2?weeks after implantation, and bone matrix proteins (osteopontin, OPN; osteocalcin, OC; dentin matrix protein 1, DMP1) were distributed in newly formed bone in a way comparable to normal bone. Some resorption cavities containing osteoclasts were also present. By 3?weeks after implantation, most of the implanted atelocollagen was replaced by new bone containing many resorption cavities, and OPN, OC, and DMP1 were deposited in the residual collagenous matrix. After 4?weeks, nearly all of the atelocollagen implant was replaced with new bone including hematopoietic marrow. Immunohistochemistry for the telopeptide region of type I collagen (TeloCOL1) during these processes demonstrated that the TeloCOL1-negative atelocollagen implant was replaced by TeloCOL1-positive collagenous matrix and new bone, indicating that new bone was mostly composed of endogenous type I collagen. These findings suggest that the atelocollagen itself can support bone regeneration by promoting osteoblast differentiation and type I collagen production.