Electron microscopic structures, serum resistance, and plasmid restructuring of New Delhi metallo-β-lactamase-1 (NDM-1)-producing ST42 Klebsiella pneumoniae emerging in Japan
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- 作者：Tatsuo Yamamoto (1)
Tomomi Takano (1)
Takehito Fusegawa (2)
Tetsuo Shibuya (2)
Wei-Chun Hung (1)
Wataru Higuchi (1)
Yasuhisa Iwao (1)
Olga Khokhlova (1)
Ivan Reva (1) - 关键词：New Delhi metallo ; β ; lactamase ; 1 (NDM ; 1) ; Klebsiella pneumoniae ; Serum resistance ; Plasmid restructuring ; Azithromycin resistance (mphA)
- 刊名：Journal of Infection and Chemotherapy
- 出版年：2013
- 出版时间：February 2013
- 年：2013
- 卷：19
- 期：1
- 页码：118-127
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Tomomi Takano (1)
Takehito Fusegawa (2)
Tetsuo Shibuya (2)
Wei-Chun Hung (1)
Wataru Higuchi (1)
Yasuhisa Iwao (1)
Olga Khokhlova (1)
Ivan Reva (1)
1. Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
2. Saitama Citizen Medical Center, Saitama, Japan - ISSN：1437-7780
文摘
Enterobacteriaceae, carrying the New Delhi metallo-β-lactamase-1 (NDM-1) gene (bla NDM-1), have emerged and posed a threat since 2006. In Japan, bla NDM-1-carrying Escherichia coli was first described in 2010. In this study, we characterized NDM-1-positive Klebsiella pneumoniae strain 419 in Japan, which was isolated from the urine of a 90-year-old Japanese patient who had never been to the Indian subcontinent. K. pneumoniae 419 belonged to ST42. It possessed a surface capsule (with untypeable capsular PCR types) and was resistant to serum killing. K. pneumoniae 419 cells were occasionally flagellated or piliated and autoaggregated. K. pneumoniae 419 was resistant to β-lactams (including carbapenems), aminoglycosides, and fluoroquinolones, and was susceptible to imipenem (or biapenem), aztreonam, polymixin B, and colistin. It possessed at least eight plasmids; of those, a 74-kb plasmid (pKPJ1) of the replicon FIIA carried bla NDM-1 and was conjugally transferred to E. coli strains, with a 71-kb transferable azithromycin-resistant (mphA +) plasmid of the replicon F (pKPJ2), as a large (145-kb) plasmid (pKPJF100) through a transposition event. In addition to bla NDM-1, pKPJ1 carried arr-2, pKPJ2 carried mphA, and pKPJF100 carried both. They were negative for the 16S rRNA methylase gene, e.g., which is frequently associated with bla NDM-1. The data demonstrate that K. pneumoniae 419 possessed virulence- and fitness-associated surface structures, was resistant to serum killing, and possessed a unique (or rare) genetic background in terms of ST type and bla NDM-1-carrying plasmid.