Detection of the first lectin with antimicrobial activity present in serum of the Amazonian fish tambaqui Colossoma macropomum
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- 作者：Elba Ver?nica Matoso Maciel Carvalho (1)
Rosiely Félix Bezerra (1)
Ranilson de Souza Bezerra (1)
Janete Magali de Araújo (2)
Athiê Jorge Guerra Santos (3)
Maria Tereza dos Santos Correia (1)
Luana Cassandra Breitenbach Barroso Coelho (1) - 关键词：Tambaqui fish ; Colossoma macropomum ; Serum lectin ; Antibacterial activity
- 刊名：Fisheries Science
- 出版年：2012
- 出版时间：July 2012
- 年：2012
- 卷：78
- 期：4
- 页码：879-887
- 全文大小：351KB
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Rosiely Félix Bezerra (1)
Ranilson de Souza Bezerra (1)
Janete Magali de Araújo (2)
Athiê Jorge Guerra Santos (3)
Maria Tereza dos Santos Correia (1)
Luana Cassandra Breitenbach Barroso Coelho (1)
1. Departamento de Bioquímica, Centro de Ciências Biológicas (CCB), Universidade Federal de Pernambuco (UFPE), Rua Prof. Nelson Chaves S/N, 50670-901, Cidade Universitária Recife, PE, Brazil
2. Departamento de Antibióticos, CCB, UFPE, Rua Prof. Artur de Sá S/N, 50670-420, Cidade Universitária Recife, PE, Brazil
3. Departamento de Pesca e Aqüicultura, Laboratório de Fisiologia de Peixes, Universidade Federal Rural de Pernambuco, Av Dom Manoel de Medeiros S/N, 52171-900, Dois Irm?os Recife, PE, Brazil
文摘
Tambaqui Colossoma macropomum is the most important Amazonian native species in South American aquaculture. Innate immunity at least partially depends on the recognition of pathogen-associated molecular patterns by receptor-recognizing pathogens (PRRs). Some PRRs have been characterized in fishes, and several studies have focused on the role of lectins in the immune system of various fishes. Lectins are proteins that specifically recognize carbohydrates and which have important biological functions. Tambaqui serum lectin (ComaSeL), which was identified on the basis of its hemagglutinating activity, was pre-purified, biochemically characterized, and used in assays of antibacterial activity against pathogenic bacteria in freshwater fishes. A study of the seasonality of this lectin was performed. Comasel activity was stable at a pH between 4.0 and 9.0 and lost 100?% of its activity at 70?°C. It recognized the carbohydrates d-galactose, 1-O-methyl-d-galactopyranoside, and d-fucose, showing antibacterial activity for Gram-negative bacteria. Its activity showed significant differences between the summer and winter (p?<?0.05, Tukey test), thereby corroborating observations that tambaqui becomes more susceptible to mortality from diseases caused by bacteria and fungi during the winter. With this information, new tools may be developed for gaining a better understanding of the role of these proteins in the immune system of the tambaqui, ultimately resulting in the improved management of this fish by pisciculturists.