Genotoxic and hematological parameters in Colossoma macropomum (Pisces, Serrasalmidae) as biomarkers for environmental impact assessment in a protected area in northeastern Brazil

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• 作者：Raimunda Nonata Fortes Carvalho Neta…
• 关键词：Freshwater fish ; Micronucleus test ; Nuclear abnormalities ; Giemsa ; Acridine orange ; Tambaqui ; Erythrocyte
• 刊名：Environmental Science and Pollution Research
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：22
• 期：20
• 页码：15994-16003
• 全文大小：660 KB
• 参考文献：Ayllón F, Garcia-Vazquez E (2001) Micronuclei and other nuclear lesions as genotoxicity indicators in rainbow trout Oncorhynchus mykiss. Ecotoxicol Environ Saf 49:221-25CrossRef
  Al-Sabti K, Metcalfe CD (1995) Fish micronuclei for assessing genotoxicity in water. Mutat Res 343:121-35CrossRef
  Amado LL, Da Rosa CE, Leite AM, Moraes L, Pires WV (2006) Biomarkers in croakers Micropogonias furnieri (Teleostei: Sciaenidae) from polluted and non-polluted areas from the Patos Lagoon estuary (Southern Brazil): evidence of genotoxic and immunological effects. Mar Pollut Bull 52:199-06CrossRef
  Andrade VM, Silva J, Silva FR, Heuser V, Dias J, Yoneama ML, Freitas TRO (2004) Fish as bioindicators to assess the effects of pollution in two southern Brazilian rivers using the comet assay and micronucleus test. Environ Mol Mutagen 44:459-68CrossRef
  Bombail V, Dennis A, Gordon E, Batty J (2001) Application of the comet and micronucleus assays to butterfish (Pholis gunnellus) erythrocytes from the Firth of Forth, Scotland. Chemosphere 44:383-92CrossRef
  Brasil (2013). Lei n° 9.985, de 18 de julho de 2000, artigo N° 15. Conceitua área de Prote??o Ambiental e dá outras providências. http://?www.?rbma.?org.?br/?rbma/?pdf/?Caderno_-8_-ed.?pdf . Accessed 22 June 2013
  Carvalho-Neta RNF (2015) áreas de Prote??o Ambiental no Maranh?o: situa??o atual e estratégias de manejo. UEMA, S?o Luís
  Carvalho-Neta RNF, Farias-Filho MS (2010) Fauna de vertebrados da área de Prote??o Ambiental do Maracan?, S?o Luís-MA. In: Carvalho-Neta RNF (ed) área de Prote??o Ambiental do Maracan?: subsídios ao manejo e à Educa??o Ambiental. Café & Lápis/FAPEMA, S?o Luís, pp 52-0
  Carvalho-Neta RNF (2010) área de Prote??o Ambiental do Maracan?: subsídios ao manejo e à Educa??o Ambiental. Café & Lápis/FAPEMA, S?o Luís
  Carvalho-Neta RNF, Torres AR Jr, Abreu-Silva AL (2012) Biomarkers in catfish Sciades herzbergii (Teleostei: Ariidae) from polluted and non-polluted areas (S?o Marcos-Bay, Northeastern Brazil). Appl Biochem Biotechnol 166:1314-327CrossRef
  Carvalho-Neta RNF, Abreu-Silva AL (2013) Glutathione S-Transferase as biomarker in Sciades herzbergii (Siluriformes: Ariidae) for environmental monitoring: the case study of S?o Marcos Bay, Maranh?o, Brazil. Brazil. Lat Am J Aquat Res 41:217-25
  Carvalho Neta RNF, Torres AR Jr, Silva D, Cortez CM (2014) A simple mathematical model based on biomarkers in stress-resistant catfish species, sciades herzbergii (Pisces, ariidae), in S?o Marcos Bay, Brazil. Appl Biochem Biotechnol 174:2380-391CrossRef
  Carrasco KR, Tilbury KL, Myers MS (1990) Assessment of the piscine micronucleus test as an in situ biological indicator of chemical contaminant effects. Can J Fish Aquat Sci 47:2123-136CrossRef
  Chapadense PFG, Castro FJ, Almeida JA, Moron SE (2009) Toxicidade do herbicida atrazina em Colossoma macropomum. Ver Bras Saúde Prod 2:395-08
  Conama (2005) Resolu??o número 357. Diário Oficial da República Federativa do Brasil. http://?www.?mma.?gov.?br/?port/?conama/?res/?res05/?res35705.?pdf . Accessed 21 August 2013
  Conama (2011) Resolu??o número 430. Diário Oficial da República Federativa do Brasil. http://?www.?mma.?gov.?br/?port/?conama/?legiabre.?cfm??codlegi=-46 . Accessed 18 March 2015
  Ferraro MVM, Fenocchio AS, Mantovani MS, Ribeiro CDO, Cestari MM (2004) Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus as evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests. Genet Mol Biol 27:103-07CrossRef
  Flammarion P, Devaux A, Nehls S, Migeon B, Noury P (2002) Multibiomarker responses in fish from the Moselle River (France). Ecotoxicol Environ Saf 51:145-53CrossRef
  Gagnon MM, Bakhtyar S (2013) Induction of fish biomarkers by synthetic-based drilling muds. PLoS Biol 8:e69489. doi:10.-371/?journal.?pone.-069489
  Guilherme S, Duarte AC, Ahamad I, Mieiro CLH (2008) Erythrocytic nuclear abnormalities in wild and caged fish (Liza aurata) along an environmental mercury contamination gradient. Ecotoxicol Environ Saf 7:411-21CrossRef
  Gravato C, Santos MA (2002) β-Naohthiflavone liver EROD and erythrocytic nuclear abnormality induction in juvenile Dicentrarchus labrax. Ecotoxicol Environ Saf 52:69-4CrossRef
  Groff AA, Silva J, Nunes E, Ianistcki M, Guecheva TN, Oliveira AM, Oliveira CPF, Val L, Henriques JAP (2010) UVA/UVB-induced genotoxicity and lesion repair in Colossoma macropomum and Arapaima gigas Amazonian fish. J Photochem Photobiol 99:93-9CrossRef
  Heddle JA (1973) A rapid in vivo test for chromosomal damage. Mutat Res 18:187-90CrossRef
  Jesus TB, Carvalho CEV (2008) Utiliza??o de biomarcadores em peixes como ferramenta para avalia??o de contamina??o ambiental por mercúrio (Hg). Oecol Bras 12:682-93
  Lazzari R, Neto JR, Corrêia V, Rossato S, Ferreira CC, Sutili FJ, Duarte MMMF (2011) Hematologia de jundiás em resposta ao nível de proteína na dieta. Ciên Anim Bra
• 作者单位：Raimunda Nonata Fortes Carvalho Neta (1)
  Débora Batista Pinheiro Sousa (2)
  Inaldo Carvalho de Macêdo Sobrinho (3)
  Emily Yarbrough Horton (4)
  Zafira da Silva de Almeida (1)
  Lígia Tchaicka (1)
  Alana Lislea de Sousa (3)
  
  1. Department of Chemistry and Biology, State University of Maranh?o (UEMA), Campus Paulo VI, S?o Luis, Maranh?o, Brazil
  2. Postgraduate Program of Aquatic Resources and Fishery (PPGRAP/UEMA), State University of Maranh?o (UEMA), Campus Paulo VI, S?o Luis, Maranh?o, Brazil
  3. Department of Clinical Veterinary, State University of Maranh?o (UEMA), Campus Paulo VI, S?o Luis, Maranh?o, Brazil
  4. Department of Anthropology, Ecological and Environmental, University of Georgia (UGA), Athens, GA, USA
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Atmospheric Protection, Air Quality Control and Air Pollution
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Industrial Pollution Prevention
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1614-7499

文摘

Genotoxic and hematological parameters in tambaqui (Colossoma macropomum), a native freshwater fish, were used as biomarkers to assess exposure to environmental stressors within the Maracan? Protected Area of Maranh?o State, Brazil. Fish were sampled at two sites—Serena Lagoon (control) and Ambude River—on four occasions (dry and rainy season), and biometric data (length and weight) recorded and blood collected from all fish for analysis. Erythrocyte indices—mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration—were calculated. Blood samples were stained with Giemsa and acridine orange, and microscopically examined for micronuclei and morphological nuclear abnormalities. Micronuclei were observed in fish from both sites, although the frequency was significantly higher in fish from the Ambude River and morphological nuclear abnormalities were only observed in fish from the Ambude River. More morphological nuclear abnormalities and a larger number of micronuclei were observed in erythrocytes stained with acridine orange compared with those stained with Giemsa. On average, erythrocyte indices were lower in fish from the Ambude River than from the Serena Lagoon. The results confirm that genotoxic and hematological parameters in C. macropomum can be used as indicators of environmental health and could be valuable tools for monitoring environmental conditions within protected areas. Keywords Freshwater fish Micronucleus test Nuclear abnormalities Giemsa Acridine orange Tambaqui Erythrocyte