Role of Indole Production on Virulence of Vibrio cholerae Using Galleria mellonella Larvae Model
详细信息    查看全文
  • 作者:Taiyeebah Nuidate ; Natta Tansila ; Suwat Saengkerdsub…
  • 刊名:Indian Journal of Microbiology
  • 出版年:2016
  • 出版时间:September 2016
  • 年:2016
  • 卷:56
  • 期:3
  • 页码:368-374
  • 全文大小:424 KB
  • 刊物主题:Microbiology; Medical Microbiology;
  • 出版者:Springer India
  • ISSN:0973-7715
  • 卷排序:56
文摘
Cell to cell communication facilitated by chemical signals plays crucial roles in regulating various cellular functions in bacteria. Indole, one such signaling molecule has been demonstrated to control various bacterial phenotypes such as biofilm formation and virulence in diverse bacteria including Vibrio cholerae. The present study explores some key factors involved in indole production and the subsequent pathogenesis of V. cholerae. Indole production was higher at 37 °C than at 30 °C, although the growth at 37 °C was slightly higher. A positive correlation was observed between indole production and biofilm formation in V. cholerae. Maximum indole production was detected at pH 7. There was no significant difference in indole production between clinical and environmental V. cholerae isolates, although indole production in one environmental isolate was significantly different. Both growth and indole production showed relevant changes with differences in salinity. An indole negative mutant strain was constructed using transposon mutagenesis and the direct effect of indole on the virulence of V. cholerae was evaluated using Galleria mellonella larvae model. Comparison to the wild type strain, the mutant significantly reduced the mortality of G. mellonella larvae which regained its virulence after complementation with exogenous indole. A gene involved in indole production and the virulence of V. cholerae was identified.KeywordsVibrio choleraeIndoleGalleria mellonellaBiofilm

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700