Characterization of Prototheca zopfii Genotypes Isolated from Cases of Bovine Mastitis and Cow Barns in China

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• 作者：Muhammad Shahid ; Tariq Ali ; Limei Zhang ; Rongguang Hou ; Shiyao Zhang…
• 关键词：Prototheca zopfii ; Genotyping ; Phylogenetic tree ; Antibiotic susceptibility ; Bovine mastitis
• 刊名：Mycopathologia
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：181
• 期：3-4
• 页码：185-195
• 全文大小：1,180 KB
• 参考文献：1.Bozzo G, Bonerba E, Di Pinto A, Bolzoni G, Ceci E, Mottola A, Tantillo G, Terio V. Occurrence of Prototheca spp. in cow milk samples. New Microbiol. 2014;37:459–64.PubMed
  2.Melville P, Watanabe E, Benites N, Ribeiro A, Silva J, Garino F, Costa E. Evaluation of the susceptibility of Prototheca zopfii to milk pasteurization. Mycopathologia. 1999;146:79–82.CrossRef PubMed
  3.Lerche M. Eine durch Algen (Prototheca) hervorgerufene Mastitis der Kuh. Berl Munch Tierarztl Wochenschr. 1952;4:64–9.
  4.Möller A, Truyen U, Roesler U. Prototheca zopfii genotype 2: the causative agent of bovine protothecal mastitis? Vet Microbiol. 2007;120:370–4.CrossRef PubMed
  5.Marques S, Silva E, Kraft C, Carvalheira J, Videira A, Huss VA, Thompson G. Bovine mastitis associated with Prototheca blaschkeae. J Clin Microbiol. 2008;46:1941–5.CrossRef PubMed PubMedCentral
  6.Osumi T, Kishimoto Y, Kano R, Maruyama H, Onozaki M, Makimura K, Ito T, Matsubara K, Hasegawa A. Prototheca zopfii genotypes isolated from cow barns and bovine mastitis in Japan. Vet Microbiol. 2008;131:419–23.CrossRef PubMed
  7.Ricchi M, Goretti M, Branda E, Cammi G, Garbarino CA, Turchetti B, Moroni P, Arrigoni N, Buzzini P. Molecular characterization of Prototheca strains isolated from Italian dairy herds. J Dairy Sci. 2010;93:4625–31.CrossRef PubMed
  8.Jagielski T, Lassa H, Ahrholdt J, Malinowski E, Roesler U. Genotyping of bovine Prototheca mastitis isolates from Poland. Vet Microbiol. 2011;149:283–7.CrossRef PubMed
  9.Gao J, Zhang HQ, He JZ, He YH, Li SM, Hou RG, Wu QX, Gao Y, Han B. Characterization of Prototheca zopfii associated with outbreak of bovine clinical mastitis in herd of Beijing. China. Mycopathologia. 2012;173:275–81.CrossRef PubMed
  10.Jagielski T, Lagneau P. Protothecosis. A pseudofungal infection. Med Mycol. 2007;17:261–70.CrossRef
  11.Ueno R, Urano N, Suzuki M. Phylogeny of the non-photosynthetic green micro-algal genus Prototheca (Trebouxiophyceae, Chlorophyta) and related taxa inferred from SSU and LSU ribosomal DNA partial sequence data. FEMS Microbiol Lett. 2003;223:275–80.CrossRef PubMed
  12.Roesler U, Möller A, Hensel A, Baumann D, Truyen U. Diversity within the current algal species Prototheca zopfii: a proposal for two Prototheca zopfii genotypes and description of a novel species, Prototheca blaschkeae sp. nov. Int J Syst Evol Microbiol. 2006;56:1419–25.CrossRef PubMed
  13.Satoh K, Ooe K, Nagayama H, Makimura K. Prototheca cutis sp. nov., a newly discovered pathogen of protothecosis isolated from inflamed human skin. Int J Syst Evol Microbiol. 2010;60:1236–40.CrossRef PubMed
  14.Melville PA, Benites NR, Sinhorini IL, Costa EO. Susceptibility and features of the ultrastructure of Prototheca zopfii following exposure to copper sulphate, silver nitrate and chlorexidine. Mycopathologia. 2002;156:1–7.CrossRef PubMed
  15.Acha PN, Szyfres B. Zoonosis y Enfermidades Transmissibles Communes al Hombre y a los Animals. 3rd ed. Washington: Organización Panamericana de la Salud; 2003.
  16.Ahrholdt J, Murugaiyan J, Straubinger RK, Jagielski T, Roesler U. Epidemiological analysis of worldwide bovine, canine and human clinical Prototheca isolates by PCR genotyping and MALDI-TOF mass spectrometry proteomic phenotyping. Med Mycol. 2012;50:234–43.CrossRef PubMed
  17.Huth N, Wenkel RF, Roschanski N, Rösler U, Plagge L, Schöniger S. Prototheca zopfii genotype 2-induced nasal dermatitis in a cat. J Comp Pathol. 2015;. doi:10.​1016/​j.​jcpa.​2015.​02.​001 .PubMed
  18.Marques S, Silva E, Carvalheira J, Thompson G. In vitro antimicrobial susceptibility of Prototheca wickerhamii and Prototheca zopfii isolated from bovine mastitis. J Dairy Sci. 2006;89:4202–4.CrossRef PubMed
  19.Roesler U, Scholz H, Hensel A. Immunodiagnostic identification of dairy cows infected with Prototheca zopfii at various clinical stages and discrimination between infected and uninfected cows. J Clin Microbiol. 2001;39:539–43.CrossRef PubMed PubMedCentral
  20.Linares MJ, Solís F, Casal M. In vitro activity of voriconazole against Prototheca wickerhamii: comparative evaluation of Sensititre and NCCLS M27-A2 methods of detection. J Clin Microbiol. 2005;43:2520–2.CrossRef PubMed PubMedCentral
  21.Bexiga R, Cavaco L, Vilela CL. Isolation of Prototheca zopfii from bovine milk. Rev Port Cienc Vet. 2003;98:33398.
  22.Buzzini P, Turchetti B, Facelli R, Baadino R, Cavarero F, Mattaalia L, Mosso P, Martini A. First large-scale isolation of Prototheca zopfii from milk produced by dairy herds in Italy. Mycopathologia. 2004;158:427–30.CrossRef PubMed
  23.Sobukawa H, Yamaguchi S, Kano R, Ito T, Suzuki K, Onozaki M, Hasegawa A, Kamata H. Molecular typing of Prototheca zopfii from bovine mastitis in Japan. J Dairy Sci. 2012;95:4442–6.CrossRef PubMed
  24.National Mastitis Council. Procedures for collecting milk samples in microbiological procedures for the diagnosis of bovine udder infection and determination of milk quality. Madison: National Mastitis Council Inc. 2004.
  25.Liu G, Liu Y, Ali T, Ferreri M, Gao J, Chen W, Yin J, Su J, Fanning S, Han B. Molecular and phenotypic characterization of Aerococcus viridans associated with subclinical bovine mastitis. PLoS ONE. 2015. doi:10.​1371/​journal.​pone.​0125001 .CrossRef
  26.Colin M. Basic mastitis bacteriology: untangling the pathogens. Irish Vet J. 2006;59:453–9.
  27.Kumar S, Tamura K, Nei M. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform. 2004;5:150–63.CrossRef PubMed
  28.Scaccabarozzi L, Turchetti B, Buzzini P, Pisoni G, Bertocchi L, Arrigoni N, Boettcher P, Bronzo V, Moroni P. Short communication: isolation of Prototheca species strains from environmental sources in dairy herds. J Dairy Sci. 2008;91:3474–7.CrossRef PubMed
  29.Aouay A, Coppée F, Cloet S, Cuvelier P, Belayew A, Lagneau PE, Mullender C. Molecular characterization of Prototheca strains isolated from bovine mastitis. J Med Mycol. 2008;18:224–7.CrossRef
  30.Kishimoto Y, Kano R, Maruyama H, Onozaki M, Makimura K, Ito T, Matsubara K, Hasegawa A, Kamata H. 26 s rDNA-based phylogenetic investigation of Japanese cattle-associated Prototheca zopfii isolates. J Vet Med Sci. 2010;72:123–6.CrossRef PubMed
  31.Chang R, Yang Q, Liu G, Liu Y, Zheng B, Su J, Han B. Treatment with gentamicin on a murine model of Protothecal mastitis. Mycopathologia. 2013;175:241–8.CrossRef PubMed
  32.Zhao J, Liu W, Lv G, Shen Y, Wu S. Protothecosis successfully treated with amikacin combined with tetracyclines. Mycoses. 2004;47:156–8.CrossRef PubMed
  33.Jagielski T, Buzzini P, Lassa H, Malinowski E, Branda E, Turchetti B, Polleichtner A, Roesler U, Lagneau PE, Marques S, Silva E, Thompson G, Stachowiak R, Bielecki J. Multicentre Etest evaluation of in vitro activity of conventional antifungal drugs against European bovine mastitis Prototheca spp. isolates. J Antimicrob Chemother. 2012;67:1945–7.CrossRef PubMed
  
• 作者单位：Muhammad Shahid (1)
  Tariq Ali (1)
  Limei Zhang (1)
  Rongguang Hou (1)
  Shiyao Zhang (1)
  Laidi Ding (1)
  Dandan Han (1)
  Zhaoju Deng (1)
  Abdur Rahman (2)
  Bo Han (1)
  
  1. College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, People’s Republic of China
  2. Faculty of Animal and Veterinary Sciences, University of Agriculture Peshawar, Peshawar, Pakistan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Microbial Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-0832

文摘

Protothecal mastitis, caused mostly by Prototheca zopfii (P. zopfii), is increasing in dairy herds and is being reported globally. The present study was aimed at studying the epidemiology of mastitis and at molecular characterization of P. zopfii isolates from dairy herds and their surroundings in three provinces of China using microbiological, biochemical and molecular methods, and antibiotic susceptibility tests. Samples from milk (n = 620) of mastitic cows and their barns sources (n = 410) including feces, feed, bedding materials and drinking water were analyzed. Among other pathogens recovered from mastitic milk, 84 (13.5 %) of the isolates were identified as P. zopfii. All of the P. zopfii isolates recovered from milk were recognized as genotype 2, whereas 58 (73.4 %) and 21 (26.6 %) isolates from environmental sources were found to be P. zopfii genotypes 1 and 2, respectively. The isolates were susceptible to some antibiotics and antifungal agents, including amikacin (78.1 %), streptomycin (58.5 %), gentamicin (17.8 %), amphotericin B (68.6 %) and nystatin (64.4 %). Additionally, the two genotypes displayed versatile patterns of susceptibility to different antimicrobials agents. Phylogeny of the genotypes on the basis of 18S SSU rDNA and 28S SSU rDNA was also investigated. The isolates of the two genotypes separated into different clades, and no interrelationship was observed among these as shown by phylogenetic analysis. The genotype 1 isolates from cow barn sources were non-pathogenic and may not present any risk of mastitis. We conclude that P. zopfii genotype 2 might play an important role in bovine mastitis in China.