Microsatellite and mitochondrial DNA assessment of the genetic diversity of captive Saiga antelopes (Saiga tatarica) in China

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• 作者：ShaSha Zhao (15812)
  ChaoQun Xu (15812)
  Gang Liu (15812)
  ShuQiang Liu (15812)
  ChongXue Zhao (25812)
  YaXiong Cui (25812)
  DeFu Hu (15812)
  
• 关键词：Saiga tatarica ; microsatellite ; mitochondrial DNA ; genetic diversity ; bottleneck
• 刊名：Chinese Science Bulletin
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：58
• 期：18
• 页码：2163-2167
• 全文大小：551KB
• 参考文献：1. Kholodova M V, Milner-Gulland E J, Easton A J, et al. Mitochondrial DNA variation and population structure of the Critically Endangered saiga antelope / Saiga tatarica. Oryx, 2006, 40: 103-07
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• 作者单位：ShaSha Zhao (15812)
  ChaoQun Xu (15812)
  Gang Liu (15812)
  ShuQiang Liu (15812)
  ChongXue Zhao (25812)
  YaXiong Cui (25812)
  DeFu Hu (15812)
  
  15812. Laboratory of Non-invasive Research Technology for Endangered Wildlife, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China
  25812. Gansu Endangered Animal Research Center, Wuwei, 733000, China
  
• ISSN：1861-9541

文摘

To estimate the genetic diversity of the only captive Saiga antelope (Saiga tatarica) population in China, 40 umbilical cord samples were collected and mitochondrial (control region) and nuclear (microsatellite) variabilities were assessed. Both of the markers revealed low genetic variability (or high genetic homogeneity) within the population. The microsatellites yielded monitoring ranges of 2- alleles. The observed heterozygosities ranged from 0.28 to 0.83, and the expected heterozygosities were between 0.27 and 0.71. The Shannon information index (Shannon I) and Polymorphic Information Content (PIC) presented overall means of 0.87 and 0.43, respectively. The gene diversity was 0.49. We found only two haplotypes in the population, and the haplotype and nucleotide diversities were 39.1% and 1.13%, respectively. Founder events, bottlenecks and inbreeding have contributed to the low genetic variation observed in this population. Our findings revealed the extent of genetic diversity maintained in the present population and the urgency of implementing a protection plan, introducing animals from other populations to enhance saiga’s genetic variation.