Rapid development of microsatellite markers for the critically endangered Saiga (Saiga tatarica) using Illumina? Miseq next generation sequencing technology

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• 作者：Carsten Nowak (1) (2)
  Steffen Zuther (3)
  Sergey V. Leontyev (3)
  Jutta Geismar (1) (2)
  
• 关键词：Short tandem repeats ; Next generation sequencing ; Kazakhstan ; Antelopes ; Antilopinae
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：6
• 期：1
• 页码：159-162
• 全文大小：178 KB
• 作者单位：Carsten Nowak (1) (2)
  Steffen Zuther (3)
  Sergey V. Leontyev (3)
  Jutta Geismar (1) (2)
  
  1. Conservation Genetics Group, Senckenberg Research Institutes and Natural History Museums, Clamecystrasse 12, Gelnhausen, Germany
  2. Biodiversity and Climate Research Centre (BiK-F), Senckenberganlage 25, 60325, Frankfurt am Main, Germany
  3. Association for the Conservation of Biodiversity of Kazakhstan (ACBK), Beybitshilik St., 18, 406 Office, Astana, 010000, Kazakhstan
  
• ISSN：1877-7260

文摘

We developed 17 variable microsatellite markers for the threatened Saiga antelope. Using one Illumina Miseq lane resulted in 105,948 unique fragments containing a microsatellite motif. Eighty-one ordered primer pairs resulted in 26 analyzable fragments, of which 17 markers showed variability in at least one population from Kazakhstan. Number of alleles ranged from 2 to 11 and values of heterozygosity varied from 0.08 to 0.91 (HO) and 0.08 to 0.88 (HE). The markers are currently used to delineate conservation units and to help understanding annual migration dynamics in this species.