Improved Extraction of Saturated Fatty Acids but not Omega-3 Fatty Acids from Sheep Red Blood Cells Using a One-Step Extraction Procedure

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• 作者：Edward H. Clayton (12) edward.clayton@dpi.nsw.gov.au
  Catherine E. Gulliver (234)
  John W. Piltz (12)
  Robert D. Taylor (5)
  Robert J. Blake (67)
  Richard G. Meyer (1)
• 关键词：FAME – ; Methanol – ; Toluene – ; Chloroform – ; Extraction – ; Sheep – ; Red blood cells – ; Omega ; 3 – ; Omega ; 6
• 刊名：Lipids
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：47
• 期：7
• 页码：719-727
• 全文大小：323.4 KB
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• 作者单位：1. NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Pine Gully Rd, Wagga Wagga, NSW 2650, Australia2. EH Graham Centre for Agricultural Innovation, an alliance between NSW DPI and Charles Sturt University, Wagga Wagga, NSW, Australia3. School of Animal and Veterinary Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia4. Future Farm Industries CRC, The University of Western Australia, Crawley, WA 6009, Australia5. Australian Livestock Research, Charlestown Rd, Kotara South, NSW 2289, Australia6. Hunter New England Area Health Service, John Hunter Hospital, Lookout Rd, New Lambton, NSW 2305, Australia7. School of Biomedical Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Life Sciences
  Biochemistry
  Medicinal Chemistry
  Microbial Genetics and Genomics
  Nutrition
  Bioorganic Chemistry
  Medical Biochemistry
  
• 出版者：Springer Berlin Heidelberg
• ISSN：1558-9307

文摘

Several methods are available to extract total lipid and methylate fatty acids from a range of samples including red blood cells (RBC). Fatty acid analysis of human RBC can be undertaken using a two-step extraction and methylation or a combined one-step extraction and methylation procedure. The lipid composition of sheep RBC differs significantly from that of humans and may affect their extraction. The purpose of the current study was to examine the efficiency of extraction of lipid and detection of fatty acids from sheep RBC using a one-step procedure. Fatty acids were analysed using a one-step extraction and methylation procedure using methanol:toluene and acetyl chloride in comparison with a two-step procedure involving extraction of lipid using chloroform:methanol and separate methylation. Concentrations of saturated fatty acids including C16:0 and C18:0 were significantly higher (42.6 and 33.9 % respectively) following extraction using the one-step procedure compared with the two-step procedure. However, concentrations of some polyunsaturated fatty acids, including C20:5n-3 and C22:6n-3 were not significantly different between either procedure. The improved detection of fatty acids may be related to the ability of different solvents to extract different lipid fractions. The differential extraction of lipids and detection of fatty acids from sheep RBC may have important implications in studies examining the effect of dietary treatment on the possible health benefits of fatty acids.