A putative novel protein, DEPDC1B, is overexpressed in oral cancer patients, and enhanced anchorage-independent growth in oral cancer cells that is mediated by Rac1 and ERK
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- 作者：Ying-Fang Su (1) (2) (3)
Chi-Yen Liang (4)
Chih-Yang Huang (5) (6) (7)
Chih-Yu Peng (1)
Claire Chiyu Chen (8)
Ming-Cheng Lin (2)
Rong-Kai Lin (1)
Wei-Wen Lin (9)
Ming-Yung Chou (1)
Pao-Hsin Liao (1)
Jaw-Ji Yang (1)
1. Institute of Medicine ; School of Dentistry ; 402 ; Taichung ; Taiwan
2. Chung-Shan Medical University ; School of Dentistry ; 402 ; Taichung ; Taiwan
3. Department of Stomatology ; Chung-Shan Medical University Hospital ; 402 ; Taichung ; Taiwan
4. Chiayi Christian Hospital ; 600 ; Chiayi ; Taiwan
5. Graduate Institute of Chinese Medical Science ; Graduate Institute of Basic Medical Science ; 404 ; Taichung ; Taiwan
6. China Medical University ; 404 ; Taichung ; Taiwan
7. Department of Health and Nutrition Biotechnology ; Asia University ; 413 ; Taichung ; Taiwan
8. Department of Chemistry and Biochemistry ; University of California at Los Angeles ; Cardiovascular Center ; Los Angeles ; 90095 ; CA ; USA
9. Veterans General Hospital ; Taichung ; 407 ; Taiwan - 关键词：Anchorage ; independent growth ; Oral cancer ; Extracellular ; signal ; regulated kinases ; Rac1 ; DEPDC1B
- 刊名：Journal of Biomedical Science
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：21
- 期：1
- 全文大小：2,188 KB
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- 刊物主题：Biomedicine
Biomedicine - 出版者：Springer Netherlands
- ISSN：1423-0127
文摘
Background The DEP domain is a globular domain containing approximately 90 amino acids, which was first discovered in 3 proteins: Drosophila disheveled, Caenorhabditis elegans EGL-10, and mammalian Pleckstrin; hence the term, DEP. DEPDC1B is categorized as a potential Rho GTPase-activating protein. The function of the DEP domain in signal transduction pathways is not fully understood. The DEPDC1B protein exhibits the characteristic features of a signaling protein, and contains 2 conserved domains (DEP and RhoGAP) that are involved in Rho GTPase signaling. Small GTPases, such as Rac, CDC42, and Rho, regulate a multitude of cell events, including cell motility, growth, differentiation, cytoskeletal reorganization and cell cycle progression. Results In this study, we found that it was a guanine nucleotide exchange factor and induced both cell migration in a cultured embryonic fibroblast cell line and cell invasion in cancer cell lines; moreover, it was observed to promote anchorage-independent growth in oral cancer cells. We also demonstrated that DEPDC1B plays a role in regulating Rac1 translocated onto cell membranes, suggesting that DEPDC1B exerts a biological function by regulating Rac1. We examined oral cancer tissue; 6 out of 7 oral cancer tissue test samples overexpressed DEPDC1B proteins, compared with normal adjacent tissue. Conclusions DEPDC1B was a guanine nucleotide exchange factor and induced both cell migration in a cultured embryonic fibroblast cell line and cell invasion in cancer cell lines; moreover, it was observed to promote anchorage-independent growth in oral cancer cells. We also demonstrated that DEPDC1B exerts a biological function by regulating Rac1. We found that oral cancer samples overexpressed DEPDC1B proteins, compared with normal adjacent tissue. Suggest that DEPDC1B plays a role in the development of oral cancer. We revealed that proliferation was linked to a novel DEPDC1B-Rac1-ERK1/2 signaling axis in oral cancer cell lines.