Cryopreservation of Panax ginseng cells

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• 作者：A. Joshi and W.-L. Teng
• 关键词：Key words  ; Panax ginseng ; Suspension ; Cryopreservation ; Rapid freezing
• 刊名：Plant Cell Reports
• 出版年：2000
• 出版时间：October 2000
• 年：2000
• 卷：19
• 期：10
• 页码：971-977
• 全文大小：140 KB

文摘

The impacts of cryoprotectants (CP) and cell status during the growth cycle on Panax ginseng cell viability during cryopreservation were investigated. The ginseng cells used had a 5–7 times proliferation rate (compared with inoculum) in 2–3 weeks and were subcultured at 2- and 4-week intervals in liquid and on solid media, respectively. After testing various CP solutions of glycerol, dimethylsulphoxide, ethylene glycol and sucrose, a combination of 10 % (v/v) glycerol and 4 % (w/v) sucrose was selected for its least cytotoxicity and highest cell viability after thawing. With this CP solution, cells throughout the growth cycle exhibited a ‘U’-shaped fluctuation of post-thaw cell viability. The highest viability (86.5 % ) occurred during the lag phase from cells already maintained in suspension culture and then in the late exponential phase (61.7 % ); the lowest level of 15.4 % was in the mid-exponential phase. Callus freshly transferred to liquid medium showed a less obvious fluctuation pattern. The recovered cells were brown-to-reddish at first and gradually returned to a light yellow colour after several subcultures.