Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius

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• 作者：Paula Baptista ; Anabela Martins ; Maria Salomé Pais ; Rui M. Tavares and Teresa Lino-Neto
• 关键词：Castanea sativa ; Pisolithus tinctorius ; Ectomycorrhiza (ECM) ; Reactive oxygen species ; Catalase (CAT) ; Superoxide dismutase (SOD)
• 刊名：Mycorrhiza
• 出版年：2007
• 出版时间：May, 2007
• 年：2007
• 卷：17
• 期：3
• 页码：185-193
• 全文大小：428 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Plant Sciences
  Ecology
  Agriculture
  Forestry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1890

文摘

Evidence for the participation of reactive oxygen species (ROS) and antioxidant systems in ectomycorrhizal (ECM) establishment is lacking. In this paper, we evaluated ROS production and the activities of superoxide dismutase (SOD) and catalase (CAT) during the early contact of the ECM fungus Pisolithus tinctorius with the roots of Castanea sativa (chestnut tree). Roots were placed in contact with P. tinctorius mycelia, and ROS production was evaluated by determining the levels of H₂O₂ and O₂ ·? during the early stages of fungal contact. Three peaks of H₂O₂ production were detected, the first two coinciding with O₂ ·? bursts. The first H₂O₂ production peak coincided with an increase in SOD activity, whereas CAT activity seemed to be implicated in H₂O₂ scavenging. P. tinctorius growth was evaluated in the presence of P. tinctorius-elicited C. sativa crude extracts prepared during the early stages of fungal contact. Differential hyphal growth that matched the H₂O₂ production profile with a delay was detected. The result suggests that during the early stages of ECM establishment, H₂O₂ results from an inhibition of ROS-scavenging enzymes and plays a role in signalling during symbiotic establishment.