Transcriptional and post-transcriptional regulation of the jasmonate signalling pathway in response to abiotic and harvesting stress in Hevea brasiliensis

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• 作者：Julien Pirrello (1)
  Julie Leclercq (1)
  Florence Dessailly (1)
  Maryannick Rio (1)
  Piyanuch Piyatrakul (1) (2)
  Kuswanhadi Kuswanhadi (3)
  Chaorong Tang (4)
  Pascal Montoro (1)
  
  1. CIRAD ; UMR AGAP ; F-34398 ; Montpellier ; France
  2. Rubber Research Institute ; Chatuchak ; Bangkok ; 10900 ; Thailand
  3. Sembawa Research Centre ; Indonesian Rubber Research Institute ; P.O 1127 ; Palembang ; 30001 ; Indonesia
  4. Rubber Research Institute ; Chinese Academy of Tropical Agricultural Sciences ; Danzhou ; 571737 ; Hainan ; China
  
• 关键词：Latex ; Tapping panel dryness ; Jasmonic acid ; Alternative splicing ; Rubber ; Transcriptional regulation
• 刊名：BMC Plant Biology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：2,393 KB
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• 刊物主题：Plant Sciences; Agriculture; Tree Biology;
• 出版者：BioMed Central
• ISSN：1471-2229

文摘

Background Latex harvesting in Hevea brasiliensis amounts to strong abiotic stress that can cause a halt in production in the most susceptible clones. Although the role of jasmonic acid has been suggested in laticifer differentiation, its role in latex production and in the response to harvesting stress has received very little attention. Only a few key genes acting in the COI-JAZ-MYC module have been isolated and studied at transcriptional level. Results Use of a reference transcriptome obtained on rubber clone PB 260 covering a large number of tissues under different environmental conditions enabled us to identify 24 contigs implicated in the jasmonate signalling pathway in the rubber tree. An analysis of their expression profile by qPCR, combined with hierarchical clustering, suggested that the jasmonate signalling pathway is highly activated in laticifer cells and, more particularly, in the response to harvesting stress. By comparison with their genomic sequences, the existence of regulation by alternative splicing was discovered for JAZ transcripts in response to harvesting stress. Lastly, positive transcriptional regulation of the HbJAZ_1405 gene by MYC was demonstrated. Conclusion This study led to the identification of all actors of jasmonate signalling pathway and revealed a specific gene expression pattern in latex cells. In-depth analysis of this regulation showed alternative splicing that has been previously shown in Arabidopsis. Interestingly, genotypic variation was observed in Hevea clones with contrasting latex metabolism. This result suggests an involvement of jasmonate signalling pathway in latex production. The data suggest that specific variability of the JA pathway may have some major consequences for resistance to stress. The data support the hypothesis that a better understanding of transcriptional regulations of jasmonate pathway during harvesting stress, along with the use of genotypic diversity in response to such stress, can be used to improve resistance to stress and rubber production in Hevea.