Extract of Scutellaria baicalensis inhibits dengue virus replication
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- 作者：Keivan Zandi (1)
Tong-Hye Lim (2)
Nor-Aziyah Rahim (1)
Meng-Hooi Shu (1)
Boon-Teong Teoh (1)
Sing-Sin Sam (1)
Mohammed-Bashar Danlami (1)
Kim-Kee Tan (1)
Sazaly Abubakar (1) - 关键词：Infectious disease ; Antiviral ; Dengue ; Flavonoids ; Baicalein ; Scultellaria baicalensis
- 刊名：BMC Complementary and Alternative Medicine
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：13
- 期：1
- 全文大小：344KB
- 参考文献：1. Tang LI, Ling AP, Koh RY, Chye SM, Voon KG: Screening of anti-dengue activity in methanolic extracts of medicinal plants. / BMC Complement Altern Med. 2012, 12:3. CrossRef
2. Zandi K, Ramedani E, Mohammadi K, Tajbakhsh S, Deilami I, Rastian Z, Fouladvand M, Yousefi F, Farshadpour F: Evaluation of antiviral activities of curcumin derivatives against HSV-1 in Vero cell line. / Nat Prod Commun. 2010, 5:1935-938.
3. Astani A, Reichling J, Schnitzler P: Screening for antiviral activities of isolated compounds from essential oils. / Evid Based Complement Altern Med 2011, 2011:253643. CrossRef
4. Han H, He W, Wang W, Gao B: Inhibitory effect of aqueous dandelion extract on HIV-1 replication and reverse transcriptase activity. / BMC Complement Alternat Med. 2011, 11:112. CrossRef
5. He W, Han H, Wang W, Gao B: Anti-influenza virus effect of aqueous extracts from dandelion. / Virol J. 2011, 8:538. CrossRef
6. Li J, Huang H, Feng M, Zhou W, shi X, Zhou P: In vitro and in vivo anti-hepatitis B virus activities of a plant extract from Geranium carolinianum L. / Antiviral Res. 2008, 79:114-20. CrossRef
7. Yang Y, Zhang L, Fan X, Qin C, Liu J: Antiviral effect of geraniin on human enterovirus 71 in vitro and in vivo . / Bioorg Med Chem Lett. 2012, 22:2209-211. CrossRef
8. Hudson JB: Applications of the phytomedicine Echinacea purpurea (purple coneflower) in infectious diseases. / J Biomed Biotechnol 2012, 2012:769896. CrossRef
9. Chen L, Dou J, Su Z, Zhou H, Wang H, Zhou W, Guo Q, Zhou C: Synergistic activity of baicalein with ribavirin against influenza A (H1N1) virus infections in cell culture and in mice. / Antiviral Res. 2011, 91:314-20. CrossRef
10. Chingwaru W, Majinda RT, Yeboah SO, Jackson JC, Kapewangolo PT, Kandawa-Schulz M, Cencic A: Tylosema esculentum (Marama) tuber and bean extracts are strong antiviral agents against rotavirus infection. / Evid Based Complement Alternat Med. 2011, 2011:284795. CrossRef
11. Ge H, Wang YF, Xu J, Gu Q, Liu HB, Xiao PG, Zhou J, Liu Y, Yang Z, Su H: Anti-influenza agents from traditional Chinese medicine. / Nat Prod Rep. 2010, 27:1758-780. CrossRef
12. An X, Zhang AL, May BH, Lin L, Xu Y, Xue CC: Oral Chinese herbal medicine for improvement of quality of life in patients with stable chronic obstructive pulmonary disease: a systematic review. / J Alternat Complement Med. 2012, 18:731-43. CrossRef
13. Scheck AC, Perry K, Hank NC, Clark WD: Anticancer activity of extracts derived from the mature roots of S. baicalensis on human malignant brain tumor cells. / BMC Complement Alternat Med 2006, 16:27. CrossRef
14. Zhang N, Van Crombruggen K, Holtappels G, Bachert C: A herbal composition of S. Baicalensis and eleutherococcus senticosus shows potent anti-inflammatory effects in an Ex vivo human mucosal tissue model. / Evid Based Complement Alternat Med 2012, 2012:673145.
15. Li HB, Chen F, Chen F: Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant S. baicalensis by high-speed counter-current chromatography. / J Chromatogr A 2005, 1074:107-10. CrossRef
16. Zandi K, Teoh BT, Sam SS, Wong PF, Mustafa MR, Abubakar S: In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2. / J Med Plants Res. 2011, 5:5534-539.
17. Zandi K, Teoh BT, Sam SS, Wong PF, Mustafa MR, Abubakar S: Antiviral activity of four types of bioflavonoid against dengue virus type-2. / Virol J. 2011, 8:560. CrossRef
18. Zandi K, Teoh BT, Sam SS, Wong PF, Mustafa MR, Abubakar S: Novel antiviral activity of baicalein against dengue virus. / BMC Complement Alternat Med. 2012, 12:3. CrossRef
19. Gubler DJ: Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. / Trends Microbiol. 2002, 10:100-03. CrossRef
20. Kuno G: Research on dengue and dengue-like illness in east Asia and the western pacific during the first half of the 20th century. / Rev Med Virol. 2007, 17:327-41. CrossRef
21. Che P, Wang L, Li Q: The development, optimization and validation of an assay for high throughput antiviral drug screening against Dengue virus. / Int J Clin Exp Med 2009, 2:363-73.
22. Zandi K, Lani R, Wong PF, Teoh BT, Sam SS, Johari J, Mustafa MR, AbuBakar S: Flavone enhances dengue virus type-2 (NGC strain) infectivity and replication in Vero cells. / Molecules. 2012, 17:2437-445. CrossRef
23. Wong SS, Abd-Jamil J, Abubakar S: Antibody neutralization and viral virulence in recurring dengue virus type 2 outbreaks. / Viral Immunol. 2007, 20:359-68. CrossRef
24. Nagai T, Suzuki Y, Tomimori T, Yamada H: Antiviral activity of plant flavonoid, 5,7,4'-trihydroxy-8-methoxyflavone, from the roots of S. baicalensis against influenza A (H3N2) and B viruses. / Biol Pharm Bull 1995, 18:295-99. CrossRef
25. Yu K, Gong Y, Lin Z, Cheng Y: Quantitative analysis and chromatographic fingerprinting for the quality evaluation of S. baicalensis Georgi using capillary electrophoresis. / J Pharm Biomed Anal 2007, 43:540-48. CrossRef
26. Burnett BP, Jia Q, Zhao Y, Levy RM: A medicinal extract of Scutellari abaicalensis and Acacia catechu acts as a dual inhibitor of cyclooxygenase and 5-lipoxygenase to reduce inflammation. / J Med Food. 2007, 10:442-51. CrossRef
27. Lu Y, Joerger R, Wu C: Study of the chemical composition and antimicrobial activities of ethanolic extracts from roots of S. baicalensis Georgi. / J Agric Food Chem 2011, 59:10934-0942. CrossRef
28. Nagai T, Moriguchi R, Suzuki Y, Tomimori T, Yamada H: Mode of action of the anti-influenza virus activity of plant flavonoid, 5,7,4'-trihydroxy-8-methoxyflavone, from the roots of Scutellaria baicalensis . / Antiviral Res. 1995, 26:11-5. CrossRef
29. Kiat TS, Pippen R, Yusof R, Ibrahim H, Khalid N, Rahman NA: Inhibitory activity of cyclohexenyl chalcone derivatives and flavonoids of fingerroot, Boesenbergia rotunda (L.), towards dengue-2 virus NS3 protease. / Bioorg Med Chem Lett 2006, 16:3337-340. CrossRef
30. Ahn HC, Lee SY, Kim JW, Son WS, Shin CG, Lee BJ: Binding aspects of baicalein to HIV-1 integrase. / Mol Cells. 2001, 12:127-30.
31. Shinozuka K, Kikuchi Y, Nishino C, Mori A, Tawata S: Inhibitory effect of flavonoids on DNA-dependent DNA and RNA polymerases. / Experientia. 1998, 44:882-85. CrossRef
32. Nafisi S, Shadaloi A, Feizbakhsh A, Tajmir-Riahi HA: RNA binding to antioxidant flavonoids. / J Photochem Photobiol B. 2009, 94:1-. CrossRef
33. Sun Y, Bi S, Song D, Qiao C, Mu D, Zhang H: Study on the interaction mechanism between DNA and the main active components in S. baicalensis Georgi. / Sensor Actuat B 2008, 129:799-10. CrossRef
34. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/13/91/prepub - 作者单位：Keivan Zandi (1)
Tong-Hye Lim (2)
Nor-Aziyah Rahim (1)
Meng-Hooi Shu (1)
Boon-Teong Teoh (1)
Sing-Sin Sam (1)
Mohammed-Bashar Danlami (1)
Kim-Kee Tan (1)
Sazaly Abubakar (1)
1. Tropical Infectious Disease Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
2. Herbitec Sendirian Berhad, G-3-7, Plaza Damas Jalan Sri Hartamas, Sri Hartamas, Kuala Lumpur, Malaysia
文摘
Background Scutellaria baicalensis (S. baicalensis) is one of the traditional Chinese medicinal herbs that have been shown to possess many health benefits. In the present study, we evaluated the in vitro antiviral activity of aqueous extract of the roots of S. baicalensis against all the four dengue virus (DENV) serotypes. Methods Aqueous extract of S. baicalensis was prepared by microwave energy steam evaporation method (MEGHE?, and the anti-dengue virus replication activity was evaluated using the foci forming unit reduction assay (FFURA) in Vero cells. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to determine the actual dengue virus RNA copy number. The presence of baicalein, a flavonoid known to inhibit dengue virus replication was determined by mass spectrometry. Results The IC50 values for the S. baicalensis extract on Vero cells following DENV adsorption ranged from 86.59 to 95.19 μg/mL for the different DENV serotypes. The IC50 values decreased to 56.02 to 77.41 μg/mL when cells were treated with the extract at the time of virus adsorption for the different DENV serotypes. The extract showed potent direct virucidal activity against extracellular infectious virus particles with IC50 that ranged from 74.33 to 95.83 μg/mL for all DENV serotypes. Weak prophylactic effects with IC50 values that ranged from 269.9 to 369.8 μg/mL were noticed when the cells were pre-treated 2 hours prior to virus inoculation. The concentration of baicalein in the S. baicalensis extract was ~1% (1.03 μg/gm dried extract). Conclusions Our study demonstrates the in vitro anti-dengue virus replication property of S. baicalensis against all the four DENV serotypes investigated. The extract reduced DENV infectivity and replication in Vero cells. The extract was rich in baicalein, and could be considered for potential development of anti-DENV therapeutics.