Antithrombotic Peptides from Scolopendra subspinipes mutilans Hydrolysates

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• 作者：Yi Kong (1) (2)
  Shuai Li (1)
  Yu Shao (1)
  Zhi-long He (1)
  Mei-mei Chen (1)
  Xin Ming (3)
  Ji-fu Wei (4)
  
• 关键词：Scolopendra subspinipes mutilans ; Enzymatic hydrolysates ; Platelet aggregation inhibitor ; Antithrombotic peptides
• 刊名：International Journal of Peptide Research and Therapeutics
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：20
• 期：2
• 页码：245-252
• 全文大小：736 KB
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• 作者单位：Yi Kong (1) (2)
  Shuai Li (1)
  Yu Shao (1)
  Zhi-long He (1)
  Mei-mei Chen (1)
  Xin Ming (3)
  Ji-fu Wei (4)
  
  1. School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, People’s Republic of China
  2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, People’s Republic of China
  3. Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA
  4. Research Division of Clinical Pharmacology, the First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People’s Republic of China
  
• ISSN：1573-3904

文摘

The centipedes are a type of traditional Chinese herb and can treat cardiovascular diseases. In this study, we aimed to isolate bioactive peptides from the enzymatic hydrolysates of centipedes. Three novel antithrombotic peptides were isolated from centipedes hydrolysates using a combination of ultrafiltration, Sephadex G-50 column, and RP-HPLC C8 column. The molecular mass of the purified peptides are 753, 515 and 985?Da measured by Electrospray Ionization Mass Spectrometry. The primary structures of these peptides were determined to be Phe-Ser-Ala-Pro-Ala-Val-Tyr (FSAPAVY), Ile-Arg-Asp-Leu (IRDL) and Asp-Leu-Asp-His-Tyr-Ser-Phe (DLDHYSF) using Edman degradation method. All isolated peptides potently prolonged the activated partial thromboplastin time. Moreover, IRDL and DLDHYSF can inhibit platelet aggregation, prolong bleeding time and clotting time. These results indicate that the enzymatic hydrolysate of Scolopendra subspinipes mutilans may contribute to the antithrombotic activity of the centipede for treatment of cardiovascular and cerebrovascular diseases.