Enhancing xylanase production in the thermophilic fungus Myceliophthora thermophila by homologous overexpression of Mtxyr1

详细信息    查看全文

• 作者：Juan Wang ; Yaning Wu ; Yanfen Gong…
• 关键词：Thermophilic fungi ; Xylanase ; Myceliophthora thermophila ; xyr1 ; Homologous expression
• 刊名：Journal of Industrial Microbiology and Biotechnology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：42
• 期：9
• 页码：1233-1241
• 全文大小：762 KB
• 参考文献：1.Akel E, Metz B, Seiboth B, Kubicek CP (2009) Molecular regulation of arabinan and l -arabinose metabolism in Hypocrea jecorina (Trichoderma reesei). Eukaryot Cell 8:1837-844PubMed Central View Article PubMed
  2.Aro N, Saloheimo A, Ilme′n M, Penttila¨ M (2001) ACEII, a novel transcriptional activator involved in regulation of cellulase and xylanase genes of Trichoderma reesei. J Biol Chem 276(26):24309-4314View Article PubMed
  3.Bailey MJ, Biely P, Poutanen KJ (1992) Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol 23:257-70View Article
  4.Barker B, Kroll K, V?disch M, Mazurie A, Kniemeyer O, Cramer RA (2012) Transcriptomic and proteomic analyses of the Aspergillus fumigatus hypoxia response using an oxygen-controlled fermenter. BMC Genome 13:62View Article
  5.Battaglia E, Klaubauf S, Vallet J, Ribot C, Lebrun MH, de Vries RP (2013) Xlr1 is involved in the transcriptional control of the pentose catabolic pathway, but not hemi-cellulolytic enzymes in Magnaporthe oryzae. Fungal Genet Biol 57:76-4View Article PubMed
  6.Berka RM, Grigoriev IV, Otillar R, Salamov A, Grimwood J, Reid I, Ishmael N, John T, Darmond C, Moisan MC, Henrissat B, Coutinho PM, Lombard V, Natvig DO, Lindquist E, Schmutz J, Lucas S, Harris P, Powlowski J, Bellemare A, Taylor D, Butler G, de Vries RP, Allijn IE, van den Brink J, Ushinsky S, Storms R, Powell AJ, Paulsen IT, Elbourne LD, Baker SE, Magnuson J, Laboissiere S, Clutterbuck AJ, Martinez D, Wogulis M, de Leon AL, Rey MW, Tsang A (2011) Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia?terrestris. Nat Biotechnol 29(10):922-27View Article PubMed
  7.Brunner K, Lichtenauer AM, Kratochwill K, Delic M, Mach RL (2007) Xyr1 regulates xylanase but not cellulase formation in the head blight fungus Fusarium graminearum. Curr Genet 52(5-):213-20View Article PubMed
  8.Chambergo FS, Bonaccorsi ED, Ferreira AJ, Ramos AS, Ferreira JR, Abrahao-Neto J, Farah JP, El-Dorry H (2002) Elucidation of the metabolic fate of glucose in the filamentous fungus Trichoderma reesei using expressed sequence tag (EST) analysis. J Biol Chem 277(16):13983-3988View Article PubMed
  9.Furukawa T, Shida Y, Kitagami N, Mori K, Kato M, Kobayashi T, Okada H, Ogasawara W, Morikawa Y (2009) Identification of specific binding sites for Xyr1, a transcriptional activator of cellulolytic and xylanolytic genes in Trichoderma reesei. Fungal Genet Biol 46(8):564-74View Article PubMed
  10.Ghose T (1987) Measurement of cellulase activities. Pure Appl Chem 59:257-68
  11.Ilmén M, Saloheimo A, Onnela ML, Penttil? ME (1997) Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol 63(4):1298-306PubMed Central PubMed
  12.Karnaouri A, Topakas E, Antonopoulou I, Christakopoulos P (2014) Genomic insights into the fungal lignocellulolytic system of Myceliophthora thermophila. Front Microbiol 5:281PubMed Central View Article PubMed
  13.Klaubauf S, Narang HM, Post H, Zhou M, Brunner K, Mach-Aigner AR, Mach RL, Heck AJ, Altelaar AF, de Vries RP (2014) Similar is not the same: differences in the function of the (hemi-) cellulolytic regulator XlnR (Xlr1/Xyr1) in filamentous fungi. Fungal Genet Biol S1087-845(14):00130-0133. doi:10.-016/?j.?fgb.-014.-7.-07
  14.Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CS, Powlowski J, Tsang A (2014) Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila. Fungal Genet Biol 72:10-0View Article PubMed
  15.Kubicek CP, Mikus M, Schuster A, Schmoll M, Seiboth B (2009) Metabolic engineering strategies for the improvement of cellulase production by Hypocrea jecorina. Biotechnol Biofuels 2(19):1-4
  16.Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-85View Article PubMed
  17.Li AN, Xie C, Zhang J, Zhang J, Li DC (2011) Cloning, expression, and characterization of serine protease from thermophilic fungus Thermoascus aurantiacus var. levisporus. J Microbiol 49(1):121-29View Article PubMed
  18.Li DC, Li AN, Papageorgiou AC (2011) Cellulases from thermophilic fungi: recent insights and biotechnological potential. Enzyme Res. doi:10.-061/-011/-08730
  19.Li JK, Feng M, Zhang L, Zhang ZH, Pan YH (2008) Proteomics analysis of major royal jelly protein changes under different storage conditions. J Proteome Res 7(8):3339-353View Article PubMed
  20.Li JX, Wang J, Wang SW, Xing M, Yu SW, Liu G (2012) Achieving efficient protein expression in Trichoderma reesei by using strong constitutive promoters. Microb Cell Fact 11:84-3PubMed Central View Article PubMed
  21.Mach-Aigner AR, Pucher ME, Steiger MG, Bauer GE, Preis SJ, Mach RL (2008) Transcriptional regulation of Xyr1, encoding the main regulator of the xylanolytic and cellulolytic enzyme system in Hypocrea jeco
• 作者单位：Juan Wang (1)
  Yaning Wu (1)
  Yanfen Gong (2)
  Shaowen Yu (1)
  Gang Liu (1)
  
  1. Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, 518060, China
  2. Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen, 518060, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Chemistry
  Biotechnology
  Genetic Engineering
  Biochemistry
  Bioinformatics
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1476-5535

文摘

The xylanase regulator 1 protein in Myceliophthora thermophila ATCC42464 (MtXyr1) is 60?% homologous with that of Trichoderma reesei. However, MtXyr1’s regulatory role on cellulolytic and xylanolytic genes in M. thermophila is unknown. Herein, MtXyr1 was overexpressed under the control of the MtPpdc (pyruvate decarboxylase) promoter. Compared with the wild type, the extracellular xylanase activities of the transformant cultured in non-inducing and inducing media for 120?h were 25.19- and 9.04-fold higher, respectively. The Mtxyr1 mRNA level was 300-fold higher than in the wild type in corncob-containing medium. However, the filter paper activity and endoglucanase activities were unchanged in corncob-containing medium and glucose-containing medium. The different zymograms between the transformant and the wild type were analyzed and identified by mass spectrometry as three xylanases of the glycoside hydrolase (GH) family 11. Thus, overexpression of xyr1 resulted in enhanced xylanase activity in M. thermophila. Xylanase production could be improved by overexpressing Mtxyr1 in M. thermophila.