参考文献:Agnihotri A, Gupta V, Lakshmikumaran MS, Shivanna KR, Prakash S, Jagannathan V (1990) Production of Eruca-Brassica hybrids by embryo rescue. Plant Breed 104:281–289. doi:10.1111/j.1439-0523.1990.tb00437.x CrossRef Ashraf M (1994) Organic substances responsible for salt tolerance in Eruca sativa. Biol Plantarum 36:255–259. doi:10.1007/BF02921095 CrossRef Bannerot H, Boulidard L, Cauderon Y, Tempe J (1974) Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea. In: Eucarpia Meeting Cruciferae, Dundee, Scotland, pp 52–54 Bannerot H, Boulidard L, Chupeau Y (1977) Unexpected difficulties met with the radish cytoplasm. Eucarpia Crucif Newsl 2:16 Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 218:401–402. doi:10.1038/281401a0 CrossRef Bhargava SN, Shukla DN, Singh N (1980) Root- and foot-rot of Eruca sativa caused by Alternaria alternata (Fr) Keissler. Curr Sci India 49:452 Bock R, Hagemann R, Kössel H, Kudla J (1993) Tissue- and stage-specific modulation of RNA editing of the psbF and psbL transcript from spinach plastids—a new regulatory mechanism? Mol Gen Genet 240:238–244. doi:10.1007/BF00277062 PubMed CrossRef Bonhomme S, Budar F, Lancelin D, Small I, Defrance MC, Pelletier G (1992) Sequence and transcript analysis of the Nco2.5 Ogura-specific fragment correlated with cytoplasmic male sterility in Brassica hybrids. Mol Gen Genet 235:340–348. doi:10.1007/BF00279379 PubMed CrossRef Bozokalfa MK, Esiyok D, Ilbi H, Kavak S, Asçiogul TK (2011) Evaluation of phenotypic diversity and geographical variation of cultivated (Eruca sativa L.) and wild (Diplotaxis tenuifolia L.) rocket plant. Plant Genetic Resour 9:454–463. doi:10.1017/S1479262111000657 CrossRef Branca F (2008) Cauliflower and broccoli. In: Prohens J, Nuez F (eds) Handbook of plant breeding—Vegetables, vol 1. Springer, Berlin, pp 151–186 Chamola R, Balyan HS, Bhat SR (2013) Effect of alien cytoplasm and fertility restorer genes on agronomic and physiological traits of Brassica juncea (L.) Czern. Plant Breed 132:681–687. doi:10.1111/pbr.12080 CrossRef De Melo P, De Giordano L (1994a) Effect of Ogura male-sterile cytoplasm on the performance of cabbage hybrid variety. I. Vegetative characteristics. Euphytica 76:117–123. doi:10.1007/BF00024028 CrossRef De Melo P, De Giordano L (1994b) Effect of Ogura male-sterile cytoplasm on the performance of cabbage hybrid variety. II. Commercial characteristics. Euphytica 78:149–154. doi:10.007/BF00021411 CrossRef De Vos AC, Broekman R, de Almeida Guerra CC, van Rijsselberghe M, Rozema J (2013) Developing and testing new halophyte crops: a case study of salt tolerance of two species of the Brassicaceae, Diplotaxis tenuifolia and Cochlearia officinalis. Environ Exp Bot 92:154–164. doi:10.1016/j.envexpbot.2012.08.003 CrossRef Delourme R, Foisset N, Horvais R, Barret P, Champagne G, Cheung WY, Landry BS, Renard M (1998) Characterisation of the radish introgression carrying the Rfo restorer gene for the Ogu-INRA cytoplasmic male sterility in rapeseed (Brassica napus L.). Theor Appl Genet 97:129–134. doi:10.1007/s001220050876 CrossRef Dieterich JH, Braun HP, Schmitz UK (2003) Alloplasmic male sterility in Brassica napus (CMS ‘Tournefortii-Stiewe’) is associated with a special gene arrangement around a novel atp9 gene. Mol Gen Genom 269:723–731. doi:10.1007/s00438-003-0886-3 CrossRef Dong X, Kim WK, Lim YP, Kim YK, Hur Y (2013) Ogura-CMS in Chinese cabbage (Brassica rapa ssp. pekinensis) causes delayed expression of many nuclear genes. Plant Sci 199–200:7–17. doi:10.1016/j.plantsci.2012.11.001 PubMed CrossRef Egea-Gilabert C, Fernández JA, Migliaro D, Martínez-Sánchez JJ, Vicente MJ (2009) Genetic variability in wild vs. cultivated Eruca vesicaria populations as assessed by morphological, agronomical and molecular analyses. Sci Hortic 121:260–266. doi:10.1016/j.scienta.2009.02.020 CrossRef Fahleson J, Råhlén L, Glimelius K (1988) Analysis of plants regenerated from protoplast fusions between Brassica napus and Eruca sativa. Theor Appl Genet 76:507–512. doi:10.1007/BF00260900 PubMed CrossRef Geddy R, Mahé L, Brown GG (2005) Cell-specific regulation of a Brassica napus CMS-associated gene by a nuclear restorer with related effects on a floral homeotic gene promoter. Plant J 41:333–345. doi:10.1111/j.1365-323.2004.02305.x PubMed CrossRef González-Melendi P, Uyttewaal M, Morcillo CN, Mora JRH, Fajardo S, Budar F, Lucas MM (2008) A light and electron microscopy analysis of the events leading to male sterility in Ogu-INRA CMS of rapeseed (Brassica napus). J Exp Bot 59:827–838. doi:10.1093/jxb/erm365 PubMed CrossRef Gottlob-McHugh SG, Levesque M, MacKenzie K, Olson M, Yarosh O, Johnson DA (1990) Organization of the 5S rRNA genes in the soybean Glycine max (L.) Merrill and conservation of the 5S rDNA repeat structure in higher plants. Genome 33:94–100. doi:10.1139/g30-072 CrossRef Gourret JP, Delourme R, Renard M (1992) Expression of ogu cytoplasmic male sterility in cybrids of Brassica napus. Theor Appl Genet 83:549–556. doi:10.1007/BF00226898 PubMed CrossRef Heyn FW (1976) Transfer of restorer genes from Raphanus to cytoplasmic male-sterile Brassica napus. Crucif Newsl 1:15–16 Kaneko Y, Bang SW, Matsuzawa Y (2011) Raphanus. In: Kole C (ed) Wild crop relatives: genomic and breeding resources, vegetables. Springer, Berlin, pp 247–258CrossRef Kawanabe T, Ariizumi T, Kawai-Yamada M, Uchimiya H, Toriyama K (2006) Abolition of the tapetum suicide program ruins microsporogenesis. Plant Cell Physiol 47:784–787. doi:10.1093/pcp/pcj039 PubMed CrossRef Kirti PB, Banga SS, Prakash S, Chopra VL (1995) Transfer of Ogu cytoplasmic male sterility to Brassica juncea and improvement of the male sterile line through somatic cell fusion. Theor Appl Genet 91:517–521. doi:10.1007/BF00222982 PubMed CrossRef Kofer W, Glimelius K, Bonnett HT (1991) Modifications of mitochondrial DNA cause changes in floral development in homeotic-like mutants of tobacco. Plant Cell 3:759–769. doi:10.2307/3869270 PubMed PubMedCentral CrossRef Larran S, Ronco L, Mónaco C, Andreau RH (2006) First report of Peronospora parasitica on rocket (Eruca sativa) in Argentina. Australas Plant Path 35:377–378. doi:10.1071/AP06024 CrossRef Li N, Zhang DS, Liu HS, Yin CS, Li XX, Liang WQ, Yuan Z, Xu B, Chu HW, Wang J, Wen TQ, Huang H, Luo D, Ma H, Zhang DB (2006) The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development. Plant Cell 18:2999–3014. doi:10.1105/tpc.106.044107 PubMed PubMedCentral CrossRef Linke B, Nothnagel T, Börner T (1999) Morphological characterization of modified flower morphology of three novel male sterile carrot sources. Plant Breed 118:543–548. doi:10.1046/1439-0523.1999.004202.x CrossRef Liu Clarke JH, Chèvre AM, Landgren M, Glimelius K (1999) Characterization of sexual progenies of male-sterile somatic cybrids between Brassica napus and Brassica tournefortii. Theor Appl Genet 99:605–610. doi:10.1007/s001220051275 PubMed CrossRef Makaroff CA, Apel IJ, Palmer JD (1990) Characterization of radish mitochondrial atpA; influence of nuclear background on transcription of atpA-associated sequences and relationship with male sterility. Plant Mol Biol 15:735–746. doi:10.1007/BF00016123 PubMed CrossRef Matsuzawa Y, Mekiyanon S, Kaneko Y, Bang SW, Wakui K, Takahata Y (1999) Male sterility in alloplasmic Brassica rapa L. carrying Eruca sativa cytoplasm. Plant Breed 118:82–84. doi:10.1046/j.1439-0539-0523.1999.118001082.x CrossRef Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x CrossRef Navrátilová B, Bůžek J, Široký J, Havránek P (1997) Construction of intergeneric somatic hybrids between Brassica oleracea and Armoracia rusticana. Biol Plant 39:531–541. doi:10.1023/A:1000970529643 CrossRef Nishiyama I, Sarashima M, Matsuzawa Y (1991) Critical discussion on abortive interspecific crosses in Brassica. Plant Breed 107:288–302. doi:10.1111/j.1439-0523.1991.tb00552.x CrossRef Nothnagel T, Budahn H (1994) Ein CMS—assoziiertes OGURA—mtDNA Fragment in zwei selektierten Linien von Brassica oleracea var. botrytis. Vortr Pflanzenzücht 28:134–136 (in German) Ogura H (1968) Studies on the new male sterility in Japanese radish, with special references to the utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agr Kogoshima Univ 6:39–78 Ordas A, Cartea ME (2008) Cabbage and Kale. In: Prohens J, Nuez F (eds) Handbook of plant breeding—vegetables, vol 1. Springer, Berlin, pp 119–149 Paz Lima MLP, Café-Filho AC, Nogueira NL, Rossi ML, Schuta LR (2004) First report of clubroot of Eruca sativa caused by Plasmodiophora brassicae in Brazil. Plant Dis 88:573. doi:10.1094/PDIS.2004.88.5.573B CrossRef Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rousselle Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191:244–250. doi:10.1007/BF00334821 CrossRef Polowick PL, Sawhney VK (1991) Microsporogenesis in a normal line and in the ogu cytoplasmic male-sterile line of Brassica napus. Sex Plant Reprod 4:22–27. doi:10.1007/BF00202884 CrossRef Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15:8–15. doi:10.1007/BF02772108 CrossRef Prakash S, Kirti PB, Chopra VL (1995) Cytoplasmic male sterility (CMS) systems other than Ogu and Polima in Brassica—Current status. In: Proceedings of 9th International Rapeseed Congress. Cambridge, UK, pp 44–48 Renard M, Delourme R, Mesquida J, Pelletier G, Primard C, Boulidard L, Dore C, Ruffio V, Hervé Y, Morice J (1992) Male sterilities and F1 hybrids in Brassica. In: Dattée Y, Dumas C, Gallais A (eds) Reproductive biology and plant breeding. Springer, Heidelberg, pp 107–109CrossRef Schrader O, Budahn H, Ahne R (2000) Detection of 5S and 25S rRNA genes in Sinapis alba, Raphanus sativus and Brassica napus by double fluorescence in situ hybridization. Theor Appl Genet 100:665–669. doi:10.1007/s001220051337 CrossRef Sharma AK (1985) An unrecorded leaf-blight disease of taramira (Eruca sativa Mill.) from India caused by Alternaria brassicicola (Schew.) wiltshire. Curr Sci 54:942–943 (Acc. Number: WOS:A1985ARH7200017 ISSN: 0011-3891) Shinwari S, Akbar F, Rabbani MA, Mumtaz AS, Shinwari ZK (2013) Evaluation of genetic diversity in different genotypes of Eruca sativa from Pakistan by SDS-PAGE analysis. Pak J Bot 45:1235–1240 Sikdar SR, Chatterjee G, Das S, Sen SK (1990) “Erussica” the intergeneric fertile somatic hybrid developed through protoplast fusion between Eruca sativa LAM. and Brassica juncea (L.) Czern. Theor Appl Genet 79:561–567. doi:10.1007/BF00226168 PubMed CrossRef Singh R, Verma B, Prakash C, Prasad SN (1992) Effect of irrigation on yield and water-use of Indian mustard (Brassica juncea), rocket salad (Eruca sativa), safflower (Carthamus tinctorius) and pigeonpea (Cajanus cajan). Indian J Agr Sci 62:254–257 Srinivasan K, Spadaro D, Poli A, Gilardi G, Gullino ML, Garibaldi A (2012) Genetic diversity and pathogenicity of Fusarium oxysporum isolated from wilted rocket plants in Italy. Phytoparasitica 40:157–170. doi:10.1007/s12600-011-0207-z CrossRef Tu YQ, Sun J, Liu Y, Ge XH, Zhao ZG, Yao XC, Li ZY (2008) Production and characterization of intertribal somatic hybrids of Raphanus sativus and Brassica rapa with dye and medicinal plant Isatis indigotica. Plant Cell Rep 27:873–883. doi:10.1007/s00299-008-0513-1 PubMed CrossRef Verma SC, Lewis D (1977) Three gene system of sporophytic incompatibility in Eruca sativa (Cruciferae). Heredity 39:188 Verma JK, Sodhi YS, Mukhopadhyay A, Arumugam N, Gupta V, Pental D, Pradhan AK (2000) Identification of stable maintainer and fertility restorer lines for ‘Polima’ CMS in Brassica campestris. Plant Breed 119:90–92. doi:10.1046/j.1439-0523.2000.00430.x CrossRef Vizcay-Barrena G, Wilson ZA (2006) Altered tapetal PCD and pollen wall development in the Arabidopsis ms1 mutant. J Exp Bot 57:2709–2717. doi:10.1093/jxb/erl032 PubMed CrossRef Wan ZJ, Jing B, Tu JX, Ma CZ, Shen JX, Yi B, Wen J, Huang T, Wang XJ, Fu TD (2008) Genetic characterization of a new cytoplasmic male sterility system (hau) in Brassica juncea and its transfer to B. napus. Theor Appl Genet 116:355–362. doi:10.1007/s00122-007-0673-3 PubMed CrossRef Williams JGK, Kubelik AP, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18:6531–6535. doi:10.1093/nar/18.22.6531 PubMed PubMedCentral CrossRef Yamagishi H, Bhat SR (2014) Cytoplasmic male sterility in Brassicaceae crops. Breed Sci 64:38–47. doi:10.1270/jsbbs.64.38 PubMed PubMedCentral CrossRef Yamagishi H, Terachi T (1996) Molecular and biological studies on the male-sterile cytoplasm in Cruciferae. III. Distribution of Ogura-type cytoplasm among Japanese wild radishes and Asian radish cultivars. Theor Appl Genet 93:325–332. doi:10.1007/BF00223172 PubMed CrossRef Yang JH, Qi XH, Zang MF, Yu JQ (2008) MADS-box genes are associated with cytoplasmic homeosis in cytoplasmic male-sterile stem mustard as partially mimicked by specifically inhibiting mtETC. Plant Growth Regul 56:191–201. doi:10.1007/s10725-008-9300-9 CrossRef Yaniv Z, Schafferman D, Amar Z (1998) Tradition, uses and biodiversity of rocket (Eruca sativa, Brassicaceae) in Israel. Econ Bot 52:394–400. doi:10.1007/BF02862069 CrossRef
1. Institute for Breeding Research on Horticultural Crops, Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Erwin-Baur-Strasse 27, 06484, Quedlinburg, Germany 2. Department of Biology, Humboldt-University Berlin, Invalidenstrasse 42, 10115, Berlin, Germany
Key message Alloplasmic male sterile breeding lines of Eruca sativa were developed by intergeneric hybridization with CMS- Brassica oleracea, followed by recurrent backcrosses and determination of the breeding value.