Effect of a thymol application on olfactory memory and gene expression levels in the brain of the honeybee Apis mellifera

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• 作者：Elsa Bonnafé ; Florian Drouard ; Lucie Hotier…
• 关键词：Honeybee ; Olfactory conditioning ; Thymol ; TRPL ; RDL ; OA1 ; Real ; time PCR
• 刊名：Environmental Science and Pollution Research
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：22
• 期：11
• 页码：8022-8030
• 全文大小：1,068 KB
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• 作者单位：Elsa Bonnafé (1)
  Florian Drouard (2)
  Lucie Hotier (2)
  Jean-Luc Carayon (1)
  Pierre Marty (3)
  Michel Treilhou (1)
  Catherine Armengaud (2)
  
  1. VAcBio Group, EA 4357, Champollion University Center, 81012, Albi Cedex 09, France
  2. Research Center for Animal Cognition, CNRS-UMR 5169, Paul Sabatier University, 31062, Toulouse Cedex 91, France
  3. GEODE, CNRS-UMR 5602, Champollion University Center, 81012, Albi Cedex 09, France
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Atmospheric Protection, Air Quality Control and Air Pollution
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Industrial Pollution Prevention
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1614-7499

文摘

Essential oils are used by beekeepers to control the Varroa mites that infest honeybee colonies. So, bees can be exposed to thymol formulations in the hive. The effects of the monoterpenoid thymol were explored on olfactory memory and gene expression in the brain of the honeybee. In bees previously exposed to thymol (10 or 100?ng/bee), the specificity of the response to the conditioned stimulus (CS) was lost 24?h after learning. Besides, the octopamine receptor OA1 gene Amoa1 showed a significant decrease of expression 3?h after exposure with 10 or 100?ng/bee of thymol. With the same doses, expression of Rdl gene, coding for a GABA receptor subunit, was not significantly modified but the trpl gene was upregulated 1 and 24?h after exposure to thymol. These data indicated that the genes coding for the cellular targets of thymol could be rapidly regulated after exposure to this molecule. Memory and sensory processes should be investigated in bees after chronic exposure in the hive to thymol-based preparations.