Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization
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- 作者：Feifei Xu ; You Wang ; Tianqi Tao ; Dandan Song ; Xiuhua Liu
- 关键词：Calreticulin ; Human microvascular endothelial cell ; Actin acetylation ; Microwave radiation
- 刊名：Cell Stress and Chaperones
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：22
- 期：1
- 页码：87-97
- 全文大小：
- 刊物主题：Biomedicine, general; Cell Biology; Biochemistry, general; Immunology; Cancer Research; Neurosciences;
- 出版者：Springer Netherlands
- ISSN：1466-1268
- 卷排序：22
文摘
Recent work reveals that actin acetylation modification has been linked to different normal and disease processes and the effects associated with metabolic and environmental stressors. Herein, we highlight the effects of calreticulin on actin acetylation and cell injury induced by microwave radiation in human microvascular endothelial cell (HMEC). HMEC injury was induced by high-power microwave of different power density (10, 30, 60, 100 mW/cm2, for 6 min) with or without exogenous recombinant calreticulin. The cell injury was assessed by lactate dehydrogenase (LDH) activity and Cell Counting Kit-8 in culture medium, migration ability, intercellular junction, and cytoskeleton staining in HMEC. Western blotting analysis was used to detected calreticulin expression in cytosol and nucleus and acetylation of globular actin (G-actin). We found that HMEC injury was induced by microwave radiation in a dose-dependent manner. Pretreatment HMEC with calreticulin suppressed microwave radiation-induced LDH leakage and increased cell viability and improved microwave radiation-induced decrease in migration, intercellular junction, and cytoskeleton. Meanwhile, pretreatment HMEC with exogenous calreticulin upregulated the histone acetyltransferase activity and the acetylation level of G-actin and increased the fibrous actin (F-actin)/G-actin ratio. We conclude that exogenous calreticulin protects HMEC against microwave radiation-induced injury through promoting actin acetylation and polymerization.