Characterization and fine mapping of osh15(t), a novel dwarf mutant gene in rice (Oryza sativa L.)
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- 作者：Shuxiu Fan ; Xiaoyun Yao ; Jin Liu ; Xiaoyan Dong ; Ting Mao ; Jiayu Wang
- 刊名：Genes & Genomics
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：38
- 期：9
- 页码：849-856
- 全文大小：940 KB
- 刊物主题：Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Human Genetics;
- 出版者：Springer Netherlands
- ISSN：2092-9293
- 卷排序：38
文摘
Plant height is one of the most important agronomic traits of plant architecture, and also affects grain yield in rice. In this study, we obtained a novel dwarf rice mutant of japonica variety Shennong9816, designated Shennong9816d. Compared with wild-type, the Shennong9816d plant height was significantly reduced, and the tiller number significantly increased. Additionally, the mutant yield component, and the number of large and small vascular bundles were significantly decreased compared with wild-type. Genetic analysis indicated that the Shennong9816d dwarf phenotype was controlled by a recessive nuclear gene, while the plant was shown to be sensitive to gibberellic acid. Using a large F2 population derived from a cross between Shennong9816d and the indica rice variety Habataki, the osh15(t) gene was fine mapped between RM20891 and RM20898, within a physical distance of 73.78 kb. Sequencing analysis showed that Shennong9816d carries a 1 bp mutation and a 30 bp insertion in the OSH15 region. These results suggest that osh15(t) is a novel allelic mutant originally derived from japonica variety Shennong9816, which may be useful for introducing the semi-dwarf phenotype to improve plant architecture in rice breeding practice.KeywordsRiceDwarf mutantMorphological characteristicsGiberellic acidFine mapping