TLR1 expression in mouse brain was increased in a KA-induced seizure model

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• 作者：Fa-Xiang Wang ; Shi-Yong Liu ; Xin Zheng ; Xin Chen ; Lin-Xiang Lu…
• 关键词：Toll ; like receptors ; TLR1 ; Status epilepticus ; Temporal lobe epilepsy
• 刊名：Inflammation Research
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：64
• 期：7
• 页码：487-495
• 全文大小：1,713 KB
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• 作者单位：Fa-Xiang Wang (1)
  Shi-Yong Liu (1)
  Xin Zheng (1)
  Xin Chen (1)
  Lin-Xiang Lu (1)
  Bin Chen (1)
  Xiao-yi Xiong (2)
  Hai-Feng Shu (3)
  Qing-Wu Yang (2)
  Hui Yang (1)
  
  1. Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, 183 Xinqiao Main St, Shapingba District, Chongqing, 400037, China
  2. Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China
  3. Department of Neurosurgery, General Hospital of the People’s Liberation Army Chengdu Military Region, Sichuan, 610000, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Immunology
  Pharmacology and Toxicology
  Allergology
  Dermatology
  Neurology
  Rheumatology
  
• 出版者：Birkh盲user Basel
• ISSN：1420-908X

文摘

Objective Toll-like receptors (TLRs) that mediate inflammatory responses play an important role in epilepsy; however, whether TLR1 is also involved in epileptogenesis remains unclear. Thus, in this study, we investigated the extent and pattern of TLR1 expression in epileptic tissues. Methods One-hundred and thirty-two mice were intra-cerebroventricularly injected with PBS or kainic acid (KA) and were examined at 1, 3, 8 and 24?h. The expression pattern and distribution of TLR1 were examined by reverse-transcriptase polymerase chain reaction (RT-PCR), western blot analysis and immunohistochemistry staining. Results The mRNA and protein levels of TLR1 were significantly upregulated in the hippocampus and temporal cortex of epileptic mice compared with those of controls. TLR1 expression was increased as early as 1?h following KA treatment and peaked at 8 and 24?h. Immunohistochemistry staining demonstrated that TLR1 was distributed in the CA1-3, dentate gyrus and hilus regions of the hippocampus and different cortical regions. Immunofluorescent staining further revealed that TLR1 was primarily expressed in the neurons, microglia, and astrocytes of epileptogenic tissue. Significance These results demonstrate that cortical and hippocampal sub-regional expression of TLR1 is altered during epileptogenesis in a time- and location-specific manner, suggesting a close association with the process of epilepsy.