Effect of Huanglian Jiedu Decoction ( on glucose transporter 4 expression in adipose and skeletal muscle tissues of insulin resistant rats

详细信息    查看全文

• 作者：Guang Chen (1)
  Prof. Fu-er Lu (1)
  Dan Jin (1)
  Li-jun Xu (1)
  Kai-fu Wang (1)
  
• 关键词：Huanglian Jiedu Decoction ; glucose transporter 4 ; insulin resistance ; target tissue
• 刊名：Chinese Journal of Integrative Medicine
• 出版年：2007
• 出版时间：March 2007
• 年：2007
• 卷：13
• 期：1
• 页码：41-45
• 全文大小：441KB
• 参考文献：1. Mueckler M, Caruso C, Baldwin SA, et al. Sequence and structure of a human glucose transporter. Science 1985; 229: 941-45. CrossRef
  2. Bryant NJ, Govers R, James DE. Regulated transport of the glucose transporter GLUT4. Nat Rev Mol Cell Biol 2002; 3: 267-77. CrossRef
  3. Leng SH, Lu FE, Tu QN, et al. Effects of Huanglian Jiedu Decoction on blood glucose and lipids metabolisms in type II diabetic rats. Chin J Basic Med Tradit Chin Med 2003; 9(4): 43-5.
  4. Leng SH, Lu FE, et al. Therapeutic effects of berberine in impaired glucose tolerance rats and its influence on insulin secretion. Acta Pharmacol Sin 2004; 25: 496-02.
  5. Lu FE, He MK, Wang KF, Leng SH. Effects and mechanisms of berberine on hyperlipidemic and insulin resistant rats. Proceedings China Association Sci Technol 2004; 1(1): 141-44.
  6. Tremblay F, Lavigne C, Jacques H, et al. Dietary cod protein restores insulin-induces activation of phosphatidylinositol 3-kinase/AKT and GLUT4 translocation to the T-tubules in skeletal muscle of high-fat-fed obese rats. Diabetes 2003; 52: 29-7. CrossRef
  7. Wu HY, Jiang XQ, Chen XQ. Study on extraction process for Huanglianjiedu capsules. Chin Tradit Patent Med 1998; 20(11): 1-.
  8. Yuan M, Konstantopoulos N, Lee J, et al. Reversal of obesity-and diet-induced insulin resistance with salicylates or targeted disruption of IKKβ. Science 2001; 293: 1673-677. CrossRef
  9. Klip A, Ramlal T, Young DA, et al. Insulin-induced translocation of glucose transporters in rat hindlimb muscles. FEBS Lett 1987; 224: 224-30. CrossRef
  10. Harrison SA, Buxton JM, Clancy BM, et al. Evidence that erythroid-type glucose transporter intrinsic activity is modulated by cadmium treatment of mouse 3T3-L1 cells. J Biol Chem 1991; 266: 19438-9449.
  11. Lazar MA. How obesity causes diabetes: not a tall tale. Science 2005; 307: 373-75. CrossRef
  12. Lu FE, Wang ZM, Guo AQ. Exploration on the hypothesis of treating diabetes mellitus with the detoxification principle of traditional Chinese medicine. Chin J Basic Med Tradit Chin Med 2002; 8(5): 335-37.
  13. Ye AL, Lu FE, Xu LJ. et al. Effects of Huanglian Jiedu Decoction on IRS-1 expression and tyrosine phosphorylation level in skeletal muscles of the rats with insulin resistanc. J Tradit Chin Med 2005;46(9): 695-97.
  14. Somwar R, Kim DY, Sweeney G, et al. GLUT4 Translocation precedes the stimulation of glucose uptake by insulin in muscle cells: potential activation of GLUT4 via p38 mitogen-activated protein kinase. Biochem J 2001; 359(Pt 3): 639-49. CrossRef
  15. Pascoe WS, Jenkins AB, Kusunoki M, et al. Insulin action and determinants of glycaemia in a rat model of type 2 (non-insulin-dependent) diabetes mellitus. Diabetologia 1992; 35: 208-15. CrossRef
  16. Kraegen EW, Clark PW, Jenkins AB, et al. Development of muscle insulin resistance after liver insulin resistance in high-fat-fed rats. Diabetes 1991; 40: 1397-403. CrossRef
  17. Reed MJ, Meszaros K, Entes LJ, et al. A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat. Metabolism 2000;49: 1390-394. CrossRef
  18. Pascoe WS, Storlien LH. Inducement by fat feeding of basal hyperglycemia in rats with abnormal beta-cell function. Model for study of etiology and pathogenesis of NIDDM. Diabetes 1990; 39: 226-33. CrossRef
  19. Hundal RS, Petersen KF, Mayerson AB, et al. Mechanism by which high-dose aspirin improves glucose metabolism in type 2 diabetes. J Clin Invest 2002;109: 1321-326. CrossRef
  20. Furtado LM, Poon V, Klip A. GLUT4 activation: thoughts on possible mechanisms. Acta Physiol Scand 2003;178: 287-96. CrossRef
  21. Kandror KV. A long search for Glut4 activation. Sci STKE 2003; 2003: pe5. CrossRef
  
• 作者单位：Guang Chen (1)
  Prof. Fu-er Lu (1)
  Dan Jin (1)
  Li-jun Xu (1)
  Kai-fu Wang (1)
  
  1. Institute of Integrative Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China
  

文摘

Objective To investigate the effects of Huanglian Jiedu Decoction ( HLJDD) on glucose transporter 4 (GLUT4) protein expressions in insulin-resistant murine target tissues. Methods The experimental male Wistar rats were established into insulin resistant models by injecting streptozotocin (STZ 30 mg/kg) via caudal vein and feeding them with high fat high caloric diet, and randomly divided into the model group, the aspirin group and the HLJDD group. Besides, a normal group was set up for control. Changes of body weight (BW), levels of serum fasting blood glucose (FBG), serum fasting insulin (FINS) and oral glucose tolerance test (OGTT) were routinely determined. The expression of GLUT4 protein in adipose and skeletal muscle tissues before and after insulin stimulation was determined with Western blot. Results In the HLJDD group after treatment, BW and FBG got decreased, OGTT improved, and the expression and translocation of GLUT4 protein elevated obviously, either before or after insulin stimulation, as compared with those in the model group, showing significant differences respectively. Conclusion The mechanism of improving insulin resistance by HLJDD is probably associated with its effect in elevating GLUT4 protein expression and translocation in adipose and skeletal muscle tissues of insulin resistant rats.