The Native Plasmid pML21 Plays a Role in Stress Tolerance in Enterococcus faecalis ML21, as Analyzed by Plasmid Curing Using Plasmid Incompatibility

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• 作者：Fang-Lei Zuo ; Li-Li Chen ; Zhu Zeng ; Xiu-Juan Feng…
• 关键词：Enterococcus faecalis ; Native plasmid ; Incompatibility ; Plasmid curing ; Stress tolerance
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：178
• 期：3
• 页码：451-461
• 全文大小：729 KB
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• 作者单位：Fang-Lei Zuo (1)
  Li-Li Chen (1)
  Zhu Zeng (1)
  Xiu-Juan Feng (1)
  Rui Yu (1)
  Xiao-Ming Lu (2)
  Hui-Qin Ma (3)
  Shang-Wu Chen (1)
  
  1. Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road Haidian District, Beijing, 100083, People’s Republic of China
  2. Chinese National Egg Engineering Research Center, Beijing, 102115, People’s Republic of China
  3. College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

To investigate the role of the native plasmid pML21 in Enterococcus faecalis ML21’s response to abiotic stresses, the plasmid pML21 was cured based on the principle of plasmid incompatibility and segregational instability, generating E. faecalis mutant strain ML0. The mutant and the wild strains were exposed to abiotic stresses: bile salts, low pH, H₂O₂, ethanol, heat, and NaCl, and their survival rate was measured. We found that curing of pML21 lead to reduced tolerance to stress in E. faecalis ML0, especially oxidative and osmotic stress. Complementation analysis suggested that the genes from pML21 played different role in stress tolerance. The result indicated that pML21 plays a role in E. faecalis ML21’s response to abiotic stresses.