Purification and Characterization of a Lipase with High Thermostability and Polar Organic Solvent-Tolerance from Aspergillus niger AN0512

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• 作者：Guang Liu ; Songqing Hu ; Lin Li ; Yi Hou
• 关键词：Aspergillus niger ; Lipase ; Purification ; Thermostable ; Polar organic solvent ; tolerance
• 刊名：Lipids
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：50
• 期：11
• 页码：1155-1163
• 全文大小：1,119 KB
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• 作者单位：Guang Liu (1)
  Songqing Hu (1) (2)
  Lin Li (1) (2)
  Yi Hou (1) (3)
  
  1. School of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510641, Guangdong, China
  2. Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou, 510641, Guangdong, China
  3. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510641, Guangdong, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Life Sciences
  Biochemistry
  Medicinal Chemistry
  Microbial Genetics and Genomics
  Nutrition
  Bioorganic Chemistry
  Medical Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1558-9307

文摘

An extracellular lipase (EC 3.1.1.3, AN0512Lip) from Aspergillus niger AN0512 was purified and its characteristics were investigated. After the process of ammonium sulfate precipitation followed by ion-exchange chromatography and gel filtration, the purified lipase was achieved with 203.6-fold purification and 22.1 % recovery. AN0512Lip exhibited the highest activity at 50 °C and pH 5.0. It was thermostable and pH-stable, as indicated by that more than 50 % activity retained at 60 °C for 20 h and more than 90 % activity retained at pH 3.0 for 20 h, respectively. AN0512Lip activity was stimulated by some divalent metal ions (especially Cu2+, Ca2+), while greatly suppressed by EDTA, indicating that AN0512Lip was a metal-activated enzyme. Moreover, AN0512Lip exhibited high tolerance for various polar organic solvents with log P < 0.8, and the highest lipase activity (476 % of its original activity) was achieved after addition of 90 % (V/V) isopropanol to the reaction mixture. AN0512Lip also displayed 3-regiospecificity and great affinity for the long-chain fatty ester. The preliminary test showed that AN0512Lip was a candidate for enriching EPA and DHA in fish oil. All the unique properties, such as thermostability, Cu2+-dependent, 3-regiospecificity, and polar organic solvent-tolerance, indicated that AN0512Lip could have potential applications in the food industry, even in organic synthesis and the pharmaceutical industry. Keywords Aspergillus niger Lipase Purification Thermostable Polar organic solvent-tolerance