The effect of organic solvents on the viability and morphofunctional properties of rhodococcus

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• 作者：I. O. Korshunova ; O. N. Pistsova ; M. S. Kuyukina…
• 关键词：actinobacteria ; Rhodococcus ; organic solvents ; resistance ; atomic force microscopy ; confocal laser scanning microscopy ; efflux pumps
• 刊名：Applied Biochemistry and Microbiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：52
• 期：1
• 页码：43-50
• 全文大小：970 KB
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• 作者单位：I. O. Korshunova (1)
  O. N. Pistsova (2)
  M. S. Kuyukina (1) (2)
  I. B. Ivshina (1) (2)
  
  1. Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, Perm, 614081, Russia
  2. Perm State University, Perm, 614900, Russia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Microbiology
  Medical Microbiology
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3024

文摘

The effect of organic solvents on the viability and structural-mechanical (size, surface roughness, and elasticity) properties of actinobacteria of the genus Rhodococcus was studied. The dependence of the survival of Rhodococcus on the hydrophobicity of the solvent, the culture density, and the incubation medium composition was demonstrated. The most resistant strains have been selected. Using combined confocal laser scanning and atomic force microscopy, we established that exposure to solvents resulted in an increase in the relative area and roughness of the surface of living cells and a decrease in their Young’s modulus. The involvement of protonand sodium-dependent efflux pumps in the formation of solvent tolerance in Rhodococcus was demonstrated. Keywords actinobacteria Rhodococcus organic solvents resistance atomic force microscopy confocal laser scanning microscopy efflux pumps