Determination of Melatonin and Its Metabolites in Biological Fluids and Eggs Using High-Performance Liquid Chromatography with Fluorescence and Quadrupole-Orbitrap High-Resolution Mass Spectrometry

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• 作者：Bingjie Yin ; Tingting Li ; Zhen Li ; Tong Dang ; Pingli He
• 关键词：Melatonin ; Biological fluids ; Egg ; HPLC ; FLD ; UHPLC ; Q ; Orbitrap HRMS ; Metabolites
• 刊名：Food Analytical Methods
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：9
• 期：5
• 页码：1142-1149
• 全文大小：434 KB
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• 作者单位：Bingjie Yin (1)
  Tingting Li (1)
  Zhen Li (2)
  Tong Dang (1)
  Pingli He (1)
  
  1. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People’s Republic of China
  2. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

Melatonin is a hormone found in animals, plants, fungi, and bacteria, which can be used as a sleep aid and in the treatment of sleep disorders. Here, a rapid and accurate method was developed for the determination of melatonin in plasma, milk, and egg using high-performance liquid chromatography with fluorimetric detection (HPLC-FLD) and ultra high performance liquid chromatography–quadrupole-orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). Sample was extracted with methanol and cleaned by passing through hydrophilic–lipophilic balanced (HLB) solid phase extraction cartridge. The method validation results showed that recovery for melatonin was between 80.3 and 105.1 % with relative standard deviations less than 9.8 %. UHPLC-Q-Orbitrap HRMS was employed as a further means of confirmation to assure accuracy of the results. Limits of detection for melatonin were 0.13 ng g−1 and 0.14 ng mL−1 for solid and liquid samples, respectively. Thus, the proposed method can be applied to detect melatonin in milk, plasma, and egg samples. Meanwhile, the metabolites of melatonin were identified using Q-Orbitrap HRMS. The approach identified five metabolites; among them, a novel metabolite, named mono-oxygenation-demethylation-melatonin, was found in plasma.