Enzymatic Analysis of Positional Distribution of Fatty Acids in Solid Fat by 1,3-Selective Transesterification with Candida antarctica Lipase B
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- 作者：Yomi Watanabe (1)
Shinichi Sato (2)
Saiko Sera (3)
Chiemi Sato (3)
Kazuaki Yoshinaga (4)
Toshiharu Nagai (4)
Ryota Sato (5)
Hiroaki Iwasaka (5)
Tsunehiro Aki (5) - 关键词：Lipid chemistry / Lipid analysis ; Biotechnology biocatalysis
- 刊名：Journal of the American Oil Chemists' Society
- 出版年：2014
- 出版时间：August 2014
- 年：2014
- 卷：91
- 期：8
- 页码：1323-1330
- 全文大小：442 KB
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Shinichi Sato (2)
Saiko Sera (3)
Chiemi Sato (3)
Kazuaki Yoshinaga (4)
Toshiharu Nagai (4)
Ryota Sato (5)
Hiroaki Iwasaka (5)
Tsunehiro Aki (5)
1. Osaka Municipal Technical Research Institute, 1-6-50 Morinomiya, Joto-ku, Osaka, 536-8553, Japan
2. Nagoya Branch, Japan Food Research Laboratories, 4-5-13 Oosu, Naka-ku, Nagoya, 460-0011, Japan
3. Central Research Laboratory, The Nisshin OilliO Group, 1 Shinmyocho, Yokosuka, 239-0832, Japan
4. R&D, Tsukishima Foods Industry Co., Ltd, 3-17-9 Higashikasai, Edogawa-ku, Tokyo, 134-8520, Japan
5. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima, 739-8530, Japan - ISSN：1558-9331
文摘
A protocol for the analysis of the positional distribution of fatty acids (FA) in solid triacylglycerols (TAG) was developed using sn-1(3) selective alcoholysis catalyzed by immobilized Candida antarctica lipase B (CALB). One part by weight of solid fat and ten parts by weight of ethanol (99.5?%) were warmed to liquefy the fat. After adding 0.44 parts by weight of CALB, the mixture was shaken at 50?°C for 10?min then at 30?°C for 2.8?h. The recovery of 2-MAG after the 3-h transesterification reaction was ca. 85?% of the maximum theoretical yield (33?mol%), with the loss of 15?% attributable to the acyl migration from sn-2 to sn-1(3). The recovery was similar to that of the solvent-free alcoholysis of structured lipids, 1,3-dipalmitoyl, 2-oleoyl glycerol and 1,3-dioleoyl, 2-palmitoyl glycerol, conducted at 30?°C for 3?h. In contrast, the acyl migration from sn-1(3) to sn-2 was hardly observed. Because the detected acyl migration was only in the direction of sn-2 to sn-1(3), and not vice versa, it is proposed to determine the FA composition of the sn-2 position of TAG by the gas chromatographic analysis of 2-MAG fraction recovered from the enzymatic reaction mixture, and the FA composition of sn-1(3) position by a mass balance using the FA composition of TAG and of the sn-2 position as inputs. The procedure was successfully applied to palm oil and shea butter, and docosahexaenoic acid (DHA)-rich single cell oil from Aurantiochytrium sp. KH105 for the first time.