Spatiotemporal pattern of TRAF3 expression after rat spinal cord injury

详细信息    查看全文

• 作者：Ya Wu (1)
  Minqian Zheng (1)
  Siqing Wang (1)
  Changzhi Song (1)
  Chuanbin Wang (1)
  Yueping Xiao (1)
  Lei Xu (1)
  Xiaozu Xu (1)
  
• 关键词：Spinal ; Cord injury ; TRAF3 ; Neuron ; Apoptosis ; Rats
• 刊名：Journal of Molecular Histology
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：45
• 期：5
• 页码：541-553
• 全文大小：3,291 KB
• 参考文献：1. Barreiro-Iglesias A, Shifman MI (2012) Use of fluorochrome-labeled inhibitors of caspases to detect neuronal apoptosis in the whole-mounted lamprey brain after spinal cord injury. Enzyme Res 2012:835731 CrossRef
  2. Basaki Y, Ikizawa K, Kajiwara K, Yanagihara Y (2002) CD40-mediated tumor necrosis factor receptor-associated factor 3 signaling upregulates IL-4-induced germline Cepsilon transcription in a human B cell line. Arch Biochem Biophys 405:199鈥?04 CrossRef
  3. Berens HM, Tyler KL (2011) The proapoptotic Bcl-2 protein Bax plays an important role in the pathogenesis of reovirus encephalitis. J Virol 85:3858鈥?871 CrossRef
  4. Bhattarai G, Lee YH, Lee NH, Lee IK, Yun BS, Hwang PH, Yi HK (2012) Fomitoside-K from Fomitopsis nigra induces apoptosis of human oral squamous cell carcinomas (YD-10B) via mitochondrial signaling pathway. Biol Pharm Bull 35:1711鈥?719 CrossRef
  5. Ceruti S, Villa G, Genovese T, Mazzon E, Longhi R, Rosa P, Bramanti P, Cuzzocrea S, Abbracchio MP (2009) The P2Y-like receptor GPR17 as a sensor of damage and a new potential target in spinal cord injury. Brain 132:2206鈥?218 CrossRef
  6. Chen KB, Uchida K, Nakajima H, Yayama T, Hirai T, Watanabe S, Guerrero AR, Kobayashi S, Ma WY, Liu SY, Baba H (2011) Tumor necrosis factor-alpha antagonist reduces apoptosis of neurons and oligodendroglia in rat spinal cord injury. Spine (Phila Pa 1976) 36:1350鈥?358 CrossRef
  7. Chen MH, Ren QX, Yang WF, Chen XL, Lu C, Sun J (2013) Influences of HIF-lalpha on Bax/Bcl-2 and VEGF expressions in rats with spinal cord injury. Int J Clin Exp Pathol 6:2312鈥?322
  8. Cheng G, Cleary AM, Ye ZS, Hong DI, Lederman S, Baltimore D (1995) Involvement of CRAF1, a relative of TRAF, in CD40 signaling. Science 267:1494鈥?498 CrossRef
  9. Cort A, Timur M, Dursun E, Kucuksayan E, Aslan M, Ozben T (2012) Effects of N-acetylcystein on bleomycin-induced apoptosis in malignant testicular germ cell tumors. J Physiol Biochem 68:555鈥?62 CrossRef
  10. Demchenko YN, Glebov OK, Zingone A, Keats JJ, Bergsagel PL, Kuehl WM (2010) Classical and/or alternative NF-kappaB pathway activation in multiple myeloma. Blood 115:3541鈥?552 CrossRef
  11. Edwards SK, Moore CR, Liu Y, Grewal S, Covey LR, Xie P (2013) N-benzyladriamycin-14-valerate (AD 198) exhibits potent anti-tumor activity on TRAF3-deficient mouse B lymphoma and human multiple myeloma. BMC Cancer 13:481 CrossRef
  12. Elmetwali T, Young LS, Palmer DH (2010) CD40 ligand-induced carcinoma cell death: a balance between activation of TNFR-associated factor (TRAF) 3-dependent death signals and suppression of TRAF6-dependent survival signals. J Immunol 184:1111鈥?120 CrossRef
  13. Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK (2006) A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation. Cell Death Differ 13:1789鈥?801 CrossRef
  14. Gruner JA (1992) A monitored contusion model of spinal cord injury in the rat. J Neurotrauma 9:123鈥?26 discussion 126鈥?28 CrossRef
  15. Haga IR, Pechenick Jowers T, Griffiths SJ, Haas J, Beard PM (2014) TRAF2 facilitates vaccinia virus replication by promoting rapid virus entry. J Virol 88:3664鈥?677 CrossRef
  16. Hu HM, O鈥橰ourke K, Boguski MS, Dixit VM (1994) A novel RING finger protein interacts with the cytoplasmic domain of CD40. J Biol Chem 269:30069鈥?0072
  17. Huan W, Wu X, Zhang S, Zhao Y, Xu H, Wang N, Li H, Chen H, Wei H, Wang Y (2012) Spatiotemporal patterns and essential role of TNF receptor-associated factor 5 expression after rat spinal cord Injury. J Mol Histol 43:527鈥?33 CrossRef
  18. Jana A, Hogan EL, Pahan K (2009) Ceramide and neurodegeneration: susceptibility of neurons and oligodendrocytes to cell damage and death. J Neurol Sci 278:5鈥?5 CrossRef
  19. Jenkinson SR, Williams JA, Jeon H, Zhang J, Nitta T, Ohigashi I, Kruhlak M, Zuklys S, Sharrow S, Adams A, Granger L, Choi Y, Siebenlist U, Bishop GA, Hollander GA, Takahama Y, Hodes RJ (2013) TRAF3 enforces the requirement for T cell cross-talk in thymic medullary epithelial development. Proc Natl Acad Sci USA 110:21107鈥?1112 CrossRef
  20. Kaeidi A, Esmaeili-Mahani S, Abbasnejad M, Sheibani V, Rasoulian B, Hajializadeh Z, Pasban-Aliabadi H (2013) Satureja khuzestanica attenuates apoptosis in hyperglycemic PC12 cells and spinal cord of diabetic rats. J Nat Med 67:61鈥?9 CrossRef
  21. Kapoor R, Singh S, Tripathi M, Bhatnagar P, Kakkar P, Gupta KC (2014) O-hexadecyl-dextran entrapped berberine nanoparticles abrogate high glucose stress induced apoptosis in primary rat hepatocytes. PLoS One 9:e89124 CrossRef
  22. Khatun S, Chaube SK, Bhattacharyya CN (2013) p53 activation and mitochondria-mediated pathway are involved during hanging death-induced neuronal cell apoptosis in dentate gyrus region of the rat brain. Springerplus 2:407 CrossRef
  23. Kwon BK, Tetzlaff W, Grauer JN, Beiner J, Vaccaro AR (2004) Pathophysiology and pharmacologic treatment of acute spinal cord injury. Spine J 4:451鈥?64 CrossRef
  24. Lee ZH, Lee SE, Kwack K, Yeo W, Lee TH, Bae SS, Suh PG, Kim HH (2001) Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells. J Leukoc Biol 69:490鈥?96
  25. Li D, Zhang J, Huang W, Jin H, Shen A, Yang L, Liu J, Fan J, Zhou Q, Wen H, Hu Y, Cui Z (2013) Up-regulation of Smurf1 after spinal cord injury in adult rats. J Mol Histol 44:381鈥?90 CrossRef
  26. Li M, Wen H, Yan Z, Ding T, Long L, Qin H, Wang H, Zhang F (2014) Temporal-spatial expression of ENOLASE after acute spinal cord injury in adult rats. Neurosci Res 79:76鈥?2 CrossRef
  27. Lin X, Wang YJ, Li Q, Hou YY, Hong MH, Cao YL, Chi ZQ, Liu JG (2009) Chronic high-dose morphine treatment promotes SH-SY5Y cell apoptosis via c-Jun N-terminal kinase-mediated activation of mitochondria-dependent pathway. FEBS J 276:2022鈥?036 CrossRef
  28. Lin WW, Hildebrand JM, Bishop GA (2013) A complex relationship between TRAF3 and non-canonical NF-kappaB2 activation in B lymphocytes. Front Immunol 4:477 CrossRef
  29. Liu S, Chen J, Cai X, Wu J, Chen X, Wu YT, Sun L, Chen ZJ (2013) MAVS recruits multiple ubiquitin E3 ligases to activate antiviral signaling cascades. Elife 2:e00785 CrossRef
  30. Marriott JB, Clarke IA, Czajka A, Dredge K, Childs K, Man HW, Schafer P, Govinda S, Muller GW, Stirling DI, Dalgleish AG (2003) A novel subclass of thalidomide analogue with anti-solid tumor activity in which caspase-dependent apoptosis is associated with altered expression of bcl-2 family proteins. Cancer Res 63:593鈥?99
  31. Miliara S, Gkouskou KK, Sharp TV, Eliopoulos AG (2013) SUMOylation is required for optimal TRAF3 signaling capacity. PLoS One 8:e80470 CrossRef
  32. Okuno S, Saito A, Hayashi T, Chan PH (2004) The c-Jun N-terminal protein kinase signaling pathway mediates Bax activation and subsequent neuronal apoptosis through interaction with Bim after transient focal cerebral ischemia. J Neurosci 24:7879鈥?887 CrossRef
  33. Rahman MA, Kim NH, Yang H, Huh SO (2012) Angelicin induces apoptosis through intrinsic caspase-dependent pathway in human SH-SY5Y neuroblastoma cells. Mol Cell Biochem 369:95鈥?04 CrossRef
  34. Saadoun S, Bell BA, Verkman AS, Papadopoulos MC (2008) Greatly improved neurological outcome after spinal cord compression injury in AQP4-deficient mice. Brain 131:1087鈥?098 CrossRef
  35. Sasaki Y, Calado DP, Derudder E, Zhang B, Shimizu Y, Mackay F, Nishikawa S, Rajewsky K, Schmidt-Supprian M (2008) NIK overexpression amplifies, whereas ablation of its TRAF3-binding domain replaces BAFF: BAFF-R-mediated survival signals in B cells. Proc Natl Acad Sci USA 105:10883鈥?0888 CrossRef
  36. Shen A, Liu Y, Zhao J, Qin J, Shi S, Chen M, Gao S, Xiao F, Lu Q, Cheng C (2008) Temporal-spatial expressions of p27kip1 and its phosphorylation on Serine-10 after acute spinal cord injury in adult rat: implications for post-traumatic glial proliferation. Neurochem Int 52:1266鈥?275 CrossRef
  37. Song Z, Jin R, Yu S, Rivet JJ, Smyth SS, Nanda A, Granger DN, Li G (2011) CD40 is essential in the upregulation of TRAF proteins and NF-kappaB-dependent proinflammatory gene expression after arterial injury. PLoS One 6:e23239 CrossRef
  38. Su X, Zhu CL, Shi W, Ni LC, Shen JH, Chen J (2012) Transient global cerebral ischemia induces up-regulation of MLTKalpha in hippocampal CA1 neurons. J Mol Histol 43:187鈥?93 CrossRef
  39. Sun B, Gao Y, Lou D, Wu X, Wei H, Wen H, Deng X, Zhang F (2013) Expression of G-protein-coupled receptor kinase 6 (GRK6) after acute spinal cord injury in adult rat. J Mol Histol 44:259鈥?70 CrossRef
  40. Sunkaria A, Wani WY, Sharma DR, Gill KD (2012) Dichlorvos exposure results in activation induced apoptotic cell death in primary rat microglia. Chem Res Toxicol 25:1762鈥?770 CrossRef
  41. Thurau M, Everett H, Tapernoux M, Tschopp J, Thome M (2006) The TRAF3-binding site of human molluscipox virus FLIP molecule MC159 is critical for its capacity to inhibit Fas-induced apoptosis. Cell Death Differ 13:1577鈥?585 CrossRef
  42. Torres BB, Caldeira FM, Gomes MG, Serakides R, de Marco Viott A, Bertagnolli AC, Fukushima FB, de Oliveira KM, Gomes MV, de Melo EG (2010) Effects of dantrolene on apoptosis and immunohistochemical expression of NeuN in the spinal cord after traumatic injury in rats. Int J Exp Pathol 91:530鈥?36 CrossRef
  43. Tsukamoto N, Kobayashi N, Azuma S, Yamamoto T, Inoue J (1999) Two differently regulated nuclear factor kappaB activation pathways triggered by the cytoplasmic tail of CD40. Proc Natl Acad Sci USA 96:1234鈥?239 CrossRef
  44. Tsuruta F, Sunayama J, Mori Y, Hattori S, Shimizu S, Tsujimoto Y, Yoshioka K, Masuyama N, Gotoh Y (2004) JNK promotes Bax translocation to mitochondria through phosphorylation of 14-3-3 proteins. EMBO J 23:1889鈥?899 CrossRef
  45. Valente AJ, Yoshida T, Clark RA, Delafontaine P, Siebenlist U, Chandrasekar B (2013) Advanced oxidation protein products induce cardiomyocyte death via Nox2/Rac1/superoxide-dependent TRAF3IP2/JNK signaling. Free Radic Biol Med 60:125鈥?35 CrossRef
  46. Wisniewski ML, Werner BG, Hom LG, Anguish LJ, Coffey CM, Parker JS (2011) Reovirus infection or ectopic expression of outer capsid protein micro1 induces apoptosis independently of the cellular proapoptotic proteins Bax and Bak. J Virol 85:296鈥?04 CrossRef
  47. Wu J, Kharebava G, Piao C, Stoica BA, Dinizo M, Sabirzhanov B, Hanscom M, Guanciale K, Faden AI (2012) Inhibition of E2F1/CDK1 pathway attenuates neuronal apoptosis in vitro and confers neuroprotection after spinal cord injury in vivo. PLoS One 7:e42129 CrossRef
  48. Xie P (2013) TRAF molecules in cell signaling and in human diseases. J Mol Signal 8:7 CrossRef
  49. Xie P, Poovassery J, Stunz LL, Smith SM, Schultz ML, Carlin LE, Bishop GA (2011) Enhanced toll-like receptor (TLR) responses of TNFR-associated factor 3 (TRAF3)-deficient B lymphocytes. J Leukoc Biol 90:1149鈥?157 CrossRef
  50. Xu D, Cui S, Sun Y, Bao G, Li W, Liu W, Zhu X, Fan J, Wang Y, Cui Z (2011) Overexpression of glucose-regulated protein 94 after spinal cord injury in rats. J Neurol Sci 309:141鈥?47 CrossRef
  51. Yin X, Yin Y, Cao FL, Chen YF, Peng Y, Hou WG, Sun SK, Luo ZJ (2012) Tanshinone IIA attenuates the inflammatory response and apoptosis after traumatic injury of the spinal cord in adult rats. PLoS One 7:e38381 CrossRef
  52. Yu X, Zhong Y, Zhu Z, Wu T, Shen A, Huang Y (2012) Increased expression of nitric oxide synthase interacting protein (NOSIP) following traumatic spinal cord injury in rats. J Mol Histol 43:661鈥?68 CrossRef
  53. Zapata JM, Llobet D, Krajewska M, Lefebvre S, Kress CL, Reed JC (2009) Lymphocyte-specific TRAF3 transgenic mice have enhanced humoral responses and develop plasmacytosis, autoimmunity, inflammation, and cancer. Blood 113:4595鈥?603 CrossRef
  54. Zarnescu O, Brehar FM, Chivu M, Ciurea AV (2008) Immunohistochemical localization of caspase-3, caspase-9 and Bax in U87 glioblastoma xenografts. J Mol Histol 39:561鈥?69 CrossRef
  55. Zhang L, Jia L, Cui S, Shi Y, Chang A, Wang P, Zhang Z (2012) AP-2alpha-dependent regulation of Bcl-2/Bax expression affects apoptosis in the trophoblast. J Mol Histol 43:681鈥?89 CrossRef
  56. Zhang J, Cui Z, Shen A, Li W, Xu G, Bao G, Sun Y, Wang L, Gu H, Zhou Y (2013a) Upregulation of myelin and lymphocyte protein (MAL) after traumatic spinal cord injury in rats. J Mol Histol 44:125鈥?34 CrossRef
  57. Zhang J, Li D, Shen A, Mao H, Jin H, Huang W, Xu D, Fan J, Chen J, Yang L, Cui Z (2013b) Expression of RBMX after spinal cord injury in rats. J Mol Neurosci 49:417鈥?29 CrossRef
  58. Zhang S, Huan W, Wei H, Shi J, Fan J, Zhao J, Shen A, Teng H (2013c) FOXO3a/p27kip1 expression and essential role after acute spinal cord injury in adult rat. J Cell Biochem 114:354鈥?65 CrossRef
  59. Zhong B, Liu X, Wang X, Li H, Darnay BG, Lin X, Sun SC, Dong C (2013) Ubiquitin-specific protease 25 regulates TLR4-dependent innate immune responses through deubiquitination of the adaptor protein TRAF3. Sci Signal 6:ra35 CrossRef
  
• 作者单位：Ya Wu (1)
  Minqian Zheng (1)
  Siqing Wang (1)
  Changzhi Song (1)
  Chuanbin Wang (1)
  Yueping Xiao (1)
  Lei Xu (1)
  Xiaozu Xu (1)
  
  1. Department of Orthopaedics, The First People鈥檚 Hospital of Yancheng, Yancheng, 224006, Jiangsu, People鈥檚 Republic of China
  
• ISSN：1567-2387

文摘

TNF receptor associated factor 3 (TRAF3), a member of the TRAF family of intracellular signaling proteins, can directly influence the phosphorylation status and activation of c-Jun N-terminal kinase, participating in CD40-induced apoptosis in carcinoma. However, its expression profile and function are still unclear in spinal cord injury (SCI). In this study, we performed an acute spinal cord contusion injury model in adult rats and detected the dynamic change patterns of TRAF3 expression in spinal cord. Western blot and immunohistochemistry revealed a striking upregulation of TRAF3 after SCI. Double immunofluorescence staining prompted that TRAF3 immunoreactivity was found in neurons rather than astrocytes. Moreover, co-localization of TRAF3/active caspase-3 was detected in neuronal nuclei. To further investigate the function of TRAF3, a neuronal cell line PC12 was employed to establish an apoptosis model in vitro. We analyzed the association of TRAF3 with active caspase-3 on PC12 cells by western blot and immunofluorescent labeling, which was parallel with the data in vivo. Additionally, knocking TRAF3 down with siRNA demonstrated the probable pro-apoptotic role of TRAF3 in the process of neuronal apoptosis. To summarize, we firstly uncover the temporal and spatial expression changes of TRAF3 in SCI. Our data suggest that TRAF3 might be implicated in central nervous system pathophysiology after SCI.