Comparative analysis of transcription factor gene families from Papaver somniferum: identification of regulatory factors involved in benzylisoquinoline alkaloid biosynthesis

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• 作者：Parul Agarwal ; Sumya Pathak ; Deepika Lakhwani ; Parul Gupta ; Mehar Hasan Asif…
• 关键词：Benzylisoquinoline alkaloids ; Biosynthetic pathway ; Cultivars ; Papaver somniferum ; Transcription factors ; Transcriptome data
• 刊名：Protoplasma
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：253
• 期：3
• 页码：857-871
• 全文大小：1,244 KB
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• 作者单位：Parul Agarwal (1) (2)
  Sumya Pathak (1)
  Deepika Lakhwani (1) (2)
  Parul Gupta (1)
  Mehar Hasan Asif (1) (2)
  Prabodh Kumar Trivedi (1) (2)
  
  1. CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India
  2. Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Zoology
  
• 出版者：Springer Wien
• ISSN：1615-6102

文摘

Opium poppy (Papaver somniferum L.), known for biosynthesis of several therapeutically important benzylisoquinoline alkaloids (BIAs), has emerged as the premier organism to study plant alkaloid metabolism. The most prominent molecules produced in opium poppy include narcotic analgesic morphine, the cough suppressant codeine, the muscle relaxant papaverine and the anti-microbial agent sanguinarine and berberine. Despite several health benefits, biosynthesis of some of these molecules is very low due to tight temporal and spatial regulation of the genes committed to their biosynthesis. Transcription factors, one of the prime regulators of secondary plant product biosynthesis, might be involved in controlled biosynthesis of BIAs in P. somniferum. In this study, identification of members of different transcription factor gene families using transcriptome datasets of 10 cultivars of P. somniferum with distinct chemoprofile has been carried out. Analysis suggests that most represented transcription factor gene family in all the poppy cultivars is WRKY. Comparative transcriptome analysis revealed differential expression pattern of the members of a set of transcription factor gene families among 10 cultivars. Through analysis, two members of WRKY and one member of C3H gene family were identified as potential candidates which might regulate thebaine and papaverine biosynthesis, respectively, in poppy.