Identification of a Novel Rat NR2B Subunit Gene Promoter Region Variant and Its Association with Microwave-Induced Neuron Impairment

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• 作者：Li-Feng Wang ; Da-Wei Tian ; Hai-Juan Li ; Ya-Bing Gao…
• 关键词：NR2B ; Microwave ; Promoter ; Polymorphism ; Neurons ; Association
• 刊名：Molecular Neurobiology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：53
• 期：4
• 页码：2100-2111
• 全文大小：661 KB
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• 作者单位：Li-Feng Wang (1)
  Da-Wei Tian (2) (3)
  Hai-Juan Li (1)
  Ya-Bing Gao (1)
  Chang-Zhen Wang (1)
  Li Zhao (1)
  Hong-Yan Zuo (1)
  Ji Dong (1)
  Si-Mo Qiao (1)
  Yong Zou (1)
  Lu Xiong (1)
  Hong-Mei Zhou (4)
  Yue-Feng Yang (5)
  Rui-Yun Peng (1)
  Xiang-Jun Hu (1)
  
  1. Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China
  2. Vestibular Laboratory, Institute of Aviation Medicine, 28 Fucheng Road, Beijing, 100142, China
  3. Department of Aerospace Medicine Aerospace Biodynamics, The Fourth Military Medical University, 169 Changlexi Road, Xian, 100032, China
  4. Radiation Protection, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China
  5. Experimental Hematology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China
  
• 刊物主题：Neurosciences; Neurobiology; Cell Biology; Neurology;
• 出版者：Springer US
• ISSN：1559-1182

文摘

Microwave radiation has been implicated in cognitive dysfunction and neuronal injury in animal models and in human investigations; however, the mechanism of these effects is unclear. In this study, single nucleotide polymorphism (SNP) sites in the rat GRIN2B promoter region were screened. The associations of these SNPs with microwave-induced rat brain dysfunction and with rat pheochromocytoma-12 (PC12) cell function were investigated. Wistar rats (n = 160) were exposed to microwave radiation (30 mW/cm2 for 5 min/day, 5 days/week, over a period of 2 months). Screening of the GRIN2B promoter region revealed a stable C-to-T variant at nucleotide position −217 that was not induced by microwave exposure. The learning and memory ability, amino acid contents in the hippocampus and cerebrospinal fluid, and NR2B expression were then investigated in the different genotypes. Following microwave exposure, NR2B protein expression decreased, while the Glu contents in the hippocampus and CSF increased, and memory impairment was observed in the TT genotype but not the CC and CT genotypes. In PC12 cells, the effects of the T allele were more pronounced than those of the C allele on transcription factor binding ability, transcriptional activity, NR2B mRNA, and protein expression. These effects may be related to the detrimental role of the T allele and the protective role of the C allele in rat brain function and PC12 cells exposed to microwave radiation.