Genome-wide survey and expression analysis of the calcium-dependent protein kinase gene family in cassava

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• 作者：Wei Hu ; Xiaowan Hou ; Zhiqiang Xia ; Yan Yan ; Yunxie Wei…
• 关键词：Abiotic stress ; CPK ; Expression ; Response ; Signaling
• 刊名：Molecular Genetics and Genomics
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：291
• 期：1
• 页码：241-253
• 全文大小：1,727 KB
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• 作者单位：Wei Hu (1)
  Xiaowan Hou (1)
  Zhiqiang Xia (1)
  Yan Yan (1)
  Yunxie Wei (1)
  Lianzhe Wang (2)
  Meiling Zou (1)
  Cheng Lu (1)
  Wenquan Wang (1)
  Ming Peng (1)
  
  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, People’s Republic of China
  2. College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, 467000, Henan, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biochemistry
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1617-4623

文摘

Calcium-dependent protein kinases (CPKs) play important roles in regulating plant tolerance to abiotic stress and signal transduction; however, no data are currently available regarding the CPK family in cassava. Herein, we identified 27 CPK genes from cassava based on our previous genome sequencing data. Phylogenetic analysis showed that cassava CPKs could be clustered into three groups, which was further supported by gene structure and conserved protein motif analyses. Global expression analysis suggested that MeCPK genes showed distinct expression patterns in different tissues between wild subspecies and cultivated varieties, indicating their involvement in the functional diversity of different varieties. Transcriptomics, interaction networks, and co-expression assays revealed a broad transcriptional response of cassava CPKs and CPK-mediated networks to drought stress and their differential expression profiles in different varieties, implying their contribution to drought stress tolerance in cassava. Expression analysis of eight MeCPK genes suggested a comprehensive response to osmotic stress, salt, cold, abscisic acid, and H₂O₂, which indicated that cassava CPKs might be convergence points for different signaling pathways. This study provides a basis for crop improvements and understanding of abiotic stress responses and signal transduction mediated by CPKs in cassava. Keywords Abiotic stress CPK Expression Response Signaling