Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into the Islet-Like Cells: the Role of Extracellular Matrix Proteins

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• 作者：Marta Pokrywczynska…
• 关键词：Mesenchymal stem cells ; Islet ; like cells ; Collagen ; Laminin ; Vitronectin ; Transdifferentiation
• 刊名：Archivum Immunologiae et Therapiae Experimentalis
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：63
• 期：5
• 页码：377-384
• 全文大小：2,505 KB
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• 作者单位：Marta Pokrywczynska (1)
  Marzena Anna Lewandowska (2) (3)
  Sandra Krzyzanowska (1)
  Arkadiusz Jundzill (1)
  Marta Rasmus (1)
  Karolina Warda (1)
  Maciej Gagat (4)
  Aleksander Deptula (5)
  Anna Helmin-Basa (6)
  Marcin Holysz (7)
  Maciej Nowacki (1)
  Lukasz Buchholz (1)
  Magdalena Bodnar (8)
  Andrzej Marszalek (8) (9)
  Alina Grzanka (4)
  Wojciech Jozwicki (10)
  Jacek Michalkiewicz (6)
  Tomasz Drewa (1) (11)
  
  1. Chair of Regenerative Medicine, Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University in Toruń, Karlowicza 24, 85-092, Bydgoszcz, Poland
  2. Department of Molecular Oncology and Genetics, Innovative Medical Forum, Franciszek ?ukaszczyk Oncology Center, Bydgoszcz, Poland
  3. Department of Thoracic Surgery and Tumors, Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland
  4. Department of Histology and Embryology, Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland
  5. Department of Microbiology, Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland
  6. Department of Immunology, Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland
  7. Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznań, Poland
  8. Department of Clinical Pathomorphology, Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland
  9. Department of Pathology, Poznań University of Medical Sciences, Poznań, Poland
  10. Department of Tumor Pathology and Pathomorphology, Franciszek ?ukaszczyk Oncology Center, Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland
  11. Department of Urology, Nicolaus Copernicus Hospital, Toruń, Poland
  
• 刊物主题：Immunology; Pharmacology/Toxicology;
• 出版者：Springer Basel
• ISSN：1661-4917

文摘

Pancreatic islet implantation has been recently shown to be an efficient method of treatment for type 1 diabetes. However, limited availability of donor islets reduces its use. Bone morrow would provide potentially unlimited source of stem cells for generation of insulin-producing cells. This study was performed to evaluate the influence of extracellular matrix proteins like collagen, laminin, and vitronectin on bone marrow mesenchymal stem cells (BM-MSCs) transdifferentiation into islet-like cells (ILCs) in vitro. To our knowledge, this is the first report evaluating the importance of vitronectin in transdifferentiation of BM-MSCs into ILCs. Rat BM-MSCs were induced to ILCs using four-step protocol on plates coated with collagen type IV, laminin type I and vitronectin type I. Quantitative real-time PCR was performed to detect gene expression related to pancreatic β cell development. The induced cells expressed islet-related genes including: neurogenin 3, neurogenic differentiation 1, paired box 4, NK homeobox factor 6.1, glucagon, insulin 1 and insulin 2. Laminin but not collagen type IV or vitronectin enhanced expression of insulin and promoted formation of islet-like structures in monolayer culture. Laminin triggered transdifferentiation of BM-MSCs into ILCs. Keywords Mesenchymal stem cells Islet-like cells Collagen Laminin Vitronectin Transdifferentiation