Molecular breeding of transgenic white clover (Trifolium repens L.) with field resistance to Alfalfa mosaic virus through the expression of its coat protein gene
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- 作者：S. Panter (12)
P. G. Chu (3)
E. Ludlow (12)
R. Garrett (1)
R. Kalla (1)
M. Z. Z. Jahufer (1)
A. de Lucas Arbiza (124)
S. Rochfort (24)
A. Mouradov (124)
K. F. Smith (12)
G. Spangenberg (124) german.spangenberg@dpi.vic.gov.au - 关键词：Agrobacterium ; mediated plant transformation – ; Viral coat protein – ; Coat protein ; mediated virus resistance – ; Field evaluation – ; Gene flow – ; Trifolium repens
- 刊名：Transgenic Research
- 出版年：2012
- 出版时间：June 2012
- 年：2012
- 卷：21
- 期：3
- 页码：619-632
- 全文大小：487.0 KB
- 参考文献：1. Ayres JF, Murison RD, Turner AD et al (2001) A rapid semi-quantitative procedure for screening hydrocyanidic acid in white clover. Aust J Exp Agric 41:515–521
2. Barnett OW, Gibson PB (1975) Identification and prevalence of white clover mosaic virus and the resistance of Trifolium species to these viruses. Crop Sci 15:32–37
3. Baulcombe DC (1996) Mechanisms of pathogen-derived resistance to viruses in transgenic plants. Plant Cell 8:1833–1844
4. Bennett D, Morley FHW, Axelen A (1976) Bioassay responses of ewes to legume swards. Aust J Agric Res 18:495–504
5. Berardo N (1997) Prediction of the chemical composition of white clover by near-infrared reflectance spectroscopy. Grass Forage Sci 52:27–32
6. Bol JF (2005) Replication of Alfamo- and Alfamoviruses: role of the coat protein. Annu Rev Phytopathol 43:39–62
7. Brunt AA, Crabtree K, Dallwitz MJ et al (1996) Viruses of plants. CAB International, Wallingford
8. Campbell CL, Moyer JW (1984) Yield responses of six white clover clones to virus infection under field conditions. Plant Dis 68:1033–1035
9. Carlsen S, Fomsgaard I (2008) Biologically active secondary metabolites in white clover (Trifolium repens L.)—a review focusing on contents in the plant, plant-pest interactions and transformation. Chemoecol 18:129–170
10. Chen CC, Gibson PB (1972) Barriers to hybridisation of Trifolium repens with related species. Can J Genet Cytol 14:381–389
11. Crill P, Hanson EW, Hagedorn DJ (1971) Resistance and tolerance to alfalfa mosaic virus in alfalfa. Phytopathol 61:369–371
12. Davies WE (1971) Host/pollinator relationships in the evolution of herbage legumes in Britain. Sci Prog 59:573–589
13. Ding Y-L, Aldao-Humble G, Ludlow E et al (2003) Efficient plant regeneration and Agrobacterium-mediated transformation in Medicago and Trifolium species. Plant Sci 165:1419–1427
14. Emmerling M, Chu P, Smith KF et al (2004) Field evaluation of transgenic white clover with AMV immunity and development of elite transgenic germplasm. In: Hopkins A, Wang ZU, Mian R et al (eds) Molecular breeding for the genetic improvement of forage and turf: proceedings of the 3rd international symposium, molecular breeding of forage and turf. Springer, New York, pp 359–366
15. Fitchen JH, Beachy RN (1993) Genetically engineered protection against viruses in transgenic plants. Annu Rev Microbiol 47:739–763
16. Forster RLS, Musgrave DR (1985) Clover yellow vein virus in white clover (Trifolium repens) and sweet pea (Lathyrus odoratus) in the North Island of New Zealand. NZ J Agric Res 28:575–578
17. Forster RLS, Beck DL, Lough TJ (1997) Engineering for resistance to virus diseases. In: McKersie BD, Brown DCW (eds) Biotechnology and the improvement of forage legumes. CAB, Wallingford
18. Franke AA, Custer LJ, Cerna CM et al (1994) Quantitation of phytoestrogens in legumes by HPLC. J Agric Food Chem 42:1095–1913
19. Fulkerson WJ, Slack K, Hennessy DW et al (1998) Nutrients in ryegrass (Lolium spp), white clover (Trifolium repens) and kikuyu (Pennisetum clandestinum) pastures in relation to season and stage of regrowth in a subtropical environment. Aust J Exp Agric 38:227–240
20. Garrett R (1991) Impact of viruses on pasture legume productivity. In: Proceedings of Department of Agriculture Victoria white clover conference, Victoria, pp 50–57
21. George J, Dobrowolski MP, van Zijll de Jong E et al (2006) Assessment of genetic diversity in cultivars of white clover (Trifolium repens L.) detected by SSR polymorphisms. Genome 49:919–930
22. Gibson PB, Barnett OW, Skipper HD et al (1981) Effects of three viruses on growth of white clover. Plant Dis 65:50–51
23. Gibson PB, Barnett OW, Burrows PM et al (1982) Filtered-air enclosures exclude vectors and enable measurement of effects of viruses on white clover in the field. Plant Dis 66:142–144
24. Gibson PB, Barnett OW, Pederson MR et al (1989) Registration of southern regional virus resistant white clover germplasm. Crop Sci 29:241–242
25. Gilmour AR, Gogel BJ, Cullis BR et al (2002) ASReml user guide release 1. VSN, Hemel Hempstead, UK
26. Guy P, Gibbs A, Harrower K (1980) The effect of white clover mosaic virus on nodulation of white clover (Trifolium repens L. cv. Ladino). Aust J Agric Res 31:307–311
27. Harris SL, Auldist MJ, Clark DA et al (1998) Effects of white clover content in the diet on herbage intake, milk, milk production and milk composition of New Zealand dairy cows housed indoors. J Dairy Res 65:389–400
28. Jayasena KW, Hajimorad MR, Law EG et al (2001) Resistance to alfalfa mosaic virus in transgenic barrel medic lines containing the virus coat protein gene. Aust J Agric Res 52:67–72
29. Kalantidis K, Psaradakis S, Tabler M et al (2002) The occurrence of CMV-specific short RNAs in transgenic tobacco expressing virus-derived double-stranded RNA is indicative of resistance to the virus. Mol Plant Microbe Interact 15:826–833
30. Kapusta I, Janda B, Stochmal A et al (2005) Determination of saponins in aerial parts of barrel medic (Medicago truncatula) by liquid chromatography-electrospray ionization/mass spectrometry. J Agric Food Chem 53:7654–7660
31. Lechtenberg B, Schubert D, Forsbach A, Gils M, Schmidt R (2003) Neither inverted repeat T-DNA configurations nor arrangements of tandemly repeated transgenes are sufficient to trigger transgene silencing. Plant J 34:507–517
32. Lehmann J, Meister E, Gutzwiller A, Jans F, Charles JP, Blum J (1991) Should one use white clover (Trifolium repens L.) varieties rich in hydrogen cyanide? Rev Suisse d’Agric 23:107–112
33. Martin PH, Coulman BE, Peterson JF (1997) Genetics of resistance to alfalfa mosaic virus in red clover. Can J Plant Sci 77:601–605
34. McLaughlin MR (1991) A greenhouse method for aphid inoculation of alfalfa mosaic virus in white clover by co-culture of virus, vector and clover. In: Peters DC, Webster JA, Chloubers CS (eds) Aphid plant interaction: populations to molecules. Okla. Agric. Exp. Stn., Stillwater, p 318
35. Mendoza EMT, Laurena AC, Botella JR (2008) Recent advances in the development of transgenic papaya technology. Biotechnol Annu Rev 14:423–461
36. Norton MR, Johnstone GR (1998) Occurrence of alfalfa mosaic, clover yellow vein, subterranean clover red leaf, and white clover mosaic viruses in white clover throughout Australia. Aust J Agric Res 49:723–728
37. Parrella G, Lanave C, Marchoux G et al (2000) Evidence for two distinct subgroups of Alfalfa mosaic virus (AMV) from France and Italy and their relationships with other AMV strains. Arch Virol 145:2659–2667
38. Parrella G, Moretti A, Gognalons P et al (2004) The Am gene controlling resistance to alfalfa mosaic virus in tomato is located in the cluster of dominant resistance genes on chromosome 6. Phytopathology 94:345–350
39. Patterson HD, Thomson R (1971) Recovery of inter-block information when block sizes are unequal. Biometrika 58:545–554
40. Pedersen GA, McLaughlin MR (1994) Genetics of resistance to peanut stunt, clover yellow vein and alfalfa mosaic viruses in white clover. Crop Sci 34:896–900
41. Phillips GC, Collins GB, Taylor NL (1982) Interspecific hybridisation of red clover (Trifolium pratense L.) with T. sarosiense Hazsl. using in vitro embryo rescue. Theor Appl Genet 62:17–24
42. Powell AP, Sanders PR, Tumer N et al (1990) Protection against tobacco mosaic virus infection in transgenic plants requires accumulation of coat protein rather than coat protein RNA sequences. Virology 175:124–130
43. Prins M, Laimer M, Noris E et al (2008) Strategies for antiviral resistance in transgenic plants. Mol Plant Pathol 9:73–83
44. Schardl CL, Bryd AD, Benzion G et al (1987) Design and construction of a versatile system for the expression of foreign genes in plants. Gene 61:1–11
45. Smith KF, Flinn PC (1991) Monitoring the performance of a broad-based calibration for measuring the nutritive value of two independent populations of pasture using infrared reflectance (NIRS) spectroscopy. Aust J Agric Res 31:205–210
46. Spangenberg G, Wang Z-Y, Wu X et al (1995) Transgenic perennial ryegrass (Lolium perenne) plants from microprojectile bombardment of embryogenic suspension cells. Plant Sci 108:209–217
47. Tenllado F, Bol JF (2000) Genetic detection of the multiple functions of alfalfa mosaic virus coat protein in viral RNA replication, encapsidation, and movement. Virology 268:29–40
48. Tepfer M (2002) Risk assessment of virus-resistant transgenic plants. Annu Rev Phytopathol 40:467–491
49. Thomas RG (1987) Reproductive development. In: Baker MJ, Williams WM (eds) White clover. CAB, UK, pp 63–123
50. Timmerman-Vaughan GM, Pither-Joyce MD, Cooper PA et al (2001) Partial resistance of transgenic peas to alfalfa mosaic virus under greenhouse and field conditions. Crop Sci 41:846–853
51. Visschier PK, Seeley TD (1982) Foraging strategy of honeybee colonies in a temperate deciduous forest. Ecology 63:1790–1801
52. Williams WM (1987) White clover taxonomy and biosystematics. In: Baker MJ, Williams WM (eds) White clover. CAB, Wallingford, pp 323–342
53. Woodfield DR, Clifford PTP, Baird IJ et al (1994) Gene flow and estimated isolation requirements for transgenic white clover. In: Jones DD (ed) Proceedings of the 3rd international symposium on the biosafety of field tests of genetically modified plants and microorganisms. University of California, Monterey, pp 509–514
54. Xu DM, Collins GB, Hunt AG et al (1998) Resistance to alfalfa mosaic virus in transgenic burley tobacco expressing the AMV coat protein gene. Crop Sci 38:1661–1668 - 作者单位：1. Molecular Plant Breeding CRC, 1 Park Drive, Bundoora, VIC 3083, Australia2. Department of Primary Industries, Biosciences Research Division, Victorian AgriBiosciences Centre, Bundoora, VIC 3086, Australia3. CSIRO, Plant Industry, Canberra, ACT 2601, Australia4. La Trobe University, Bundoora, VIC 3086, Australia
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Biomedicine
Molecular Medicine
Plant Genetics and Genomics
Animal Genetics and Genomics
Plant Sciences
Human Genetics - 出版者：Springer Netherlands
- ISSN：1573-9368
文摘
Viral diseases, such as Alfalfa mosaic virus (AMV), cause significant reductions in the productivity and vegetative persistence of white clover plants in the field. Transgenic white clover plants ectopically expressing the viral coat protein gene encoded by the sub-genomic RNA4 of AMV were generated. Lines carrying a single copy of the transgene were analysed at the molecular, biochemical and phenotypic level under glasshouse and field conditions. Field resistance to AMV infection, as well as mitotic and meiotic stability of the transgene, were confirmed by phenotypic evaluation of the transgenic plants at two sites within Australia. The T0 and T1 generations of transgenic plants showed immunity to infection by AMV under glasshouse and field conditions, while the T4 generation in an agronomically elite ‘Grasslands Sustain’ genetic background, showed a very high level of resistance to AMV in the field. An extensive biochemical study of the T4 generation of transgenic plants, aiming to evaluate the level and composition of natural toxicants and key nutritional parameters, showed that the composition of the transgenic plants was within the range of variation seen in non-transgenic populations.