Quantification of p-cresol sulphate in human plasma by selected reaction monitoring

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• 作者：Aurora Cuoghi (1) aurora.cuoghi@unimore.it
  Marialuisa Caiazzo (1)
  Elisa Bellei (1)
  Emanuela Monari (1)
  Stefania Bergamini (1)
  Giuseppe Palladino (2)
  Tomris Ozben (3)
  Aldo Tomasi (1)
• 关键词：Biological samples &#8211 ; Process analysis &#8211 ; Mass spectrometry/ICP ; MS &#8211 ; HPLC &#8211 ; Separations/instrumentation
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：404
• 期：6-7
• 页码：2097-2104
• 全文大小：275.8 KB
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• 作者单位：1. Department of Laboratory Medicine, Medical Faculty, University Hospital of Modena and Reggio Emilia, Via del Pozzo 71, 41100 Modena, Italy2. Scientific Affairs, Bellco s.r.l., 41037 Mirandola, Modeno, Italy3. Department of Biochemistry, Medical Faculty, Akdeniz University, 07058 Antalya, Turkey
• ISSN：1618-2650

文摘

Chronic renal failure patients accumulate in the blood molecules that are normally excreted into the urine. p-Cresol Sulphate (pCS), the most representative retained toxin, shows a high level of toxicity. Therefore, its quantification could represent a prediction factor to determine the risk of endothelial dysfunction and cardiovascular complication and response to the haemodialysis treatment. The aim of this study was to evaluate the suitability of the multiple reaction monitoring (MRM) technique in order to improve the sensibility, the selectivity and the timing of pCS detection in a small amount of plasma. Deproteinized plasma of uremic patients was concentrated and dissolved in liquid chromatography (LC) mobile phase solution. pCS was quantified by LC coupled to tandem mass spectrometry (LC-MS/MS) on a triple-quadrupole mass spectrometer. Selective and sensitive detection of pCS was achieved by selecting the specific parent ion and monitoring two specific fragment ions. The MRM assay was carried out using the following transitions: m/z 187 → 80.00 and m/z 187 → 107.00. A good linearity was observed for each calibration curve. The intra-day and inter-day results showed a good precision and repeatability. The percentage recoveries indicate an optimal selectivity of the analytical method. The MRM assay to quantify pCS in a small amount of human plasma is rapid, highly sensitive, selective and with a good repeatability.