Designing a gelatin/chitosan/hyaluronic acid biopolymer using a thermophysical approach for use in tissue engineering
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- 作者：Javier Enrione (1)
Paulo Díaz-Calderón (1) (2)
Caroline R. Weinstein-Oppenheimer (3)
Elizabeth Sánchez (4)
Miguel A. Fuentes (4)
Donald I. Brown (5)
Hugo Herrera (5)
Cristian A. Acevedo (4) - 关键词：Biopolymeric scaffold ; Chitosan ; Gelatin ; Hyaluronic acid ; Thermophysical properties ; Tissue engineering
- 刊名：Bioprocess and Biosystems Engineering
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：36
- 期：12
- 页码：1947-1956
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- 作者单位：Javier Enrione (1)
Paulo Díaz-Calderón (1) (2)
Caroline R. Weinstein-Oppenheimer (3)
Elizabeth Sánchez (4)
Miguel A. Fuentes (4)
Donald I. Brown (5)
Hugo Herrera (5)
Cristian A. Acevedo (4)
1. Faculty of Medicine, School of Nutrition and Dietetics, School of Service Management, Universidad de los Andes, San Carlos de Apoquindo 2200, Las Condes, Santiago, Chile
2. Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile (USACH), Avenida Ecuador 3769, Santiago, Chile
3. Departamento de Bioquímica, Facultad de Farmacia, Universidad de Valparaíso, Avenida Gran Breta?a 1093, Valparaíso, Chile
4. Centro de Biotecnología, Universidad Técnica Federico Santa María, Avenida Espa?a 1680, Valparaíso, Chile
5. Departamento de Biología y Ciencias Ambientales, Facultad de Ciencias, Universidad de Valparaíso, Avenida Gran Breta?a 1111, Valparaíso, Chile - ISSN：1615-7605
文摘
Cell culture on biopolymeric scaffolds has provided treatments for tissue engineering. Biopolymeric mixtures based on gelatin (Ge), chitosan (Ch) and hyaluronic acid (Ha) have been used to make scaffolds for wound healing. Thermal and physical properties of scaffolds prepared with Ge, Ch and Ha were characterized. Thermal characterization was made by using differential scanning calorimetry (DSC), and physical characterization by gas pycnometry and scanning electron microscopy. The effects of Ge content and cross-linking on thermophysical properties were evaluated by means of a factorial experiment design (central composite face centered). Gelatin content was the main factor that affects the thermophysical properties (microstructure and thermal transitions) of the scaffold. The effect of Ge content of the scaffolds for tissue engineering was studied by seeding skin cells on the biopolymers. The cell attachment was not significantly modified at different Ge contents; however, the cell growth rate increased linearly with the decrease of the Ge content. This relationship together with the thermophysical characterization may be used to design scaffolds for tissue engineering.